Shono M, Shimizu I, Omoya T, Hiasa A, Honda H, Tomita Y, Ito S
General Laboratory for Medical Research, School of Medicine, University of Tokushima, Japan.
Biochem Mol Biol Int. 1998 Dec;46(5):1055-61. doi: 10.1080/15216549800204602.
To simply and directly evaluate DNA fragmentation during apoptosis induced in mouse cultured hepatocytes by an anti-Fas antibody, we examined the fluorescence intensity in cell nuclei stained with ethidium bromide and 4'-6-diamidino-2-phenylindole by optiphoto fluorescence microscopy. The intensity of the former staining for the nuclear DNA of apoptotic cells was clearly decreased compared to that of non-apoptotic cells, whereas no difference in the fluorescence intensity for the latter stain between the apoptotic and non-apoptotic groups was observed. Thus, the use of optiphoto fluorescence microscopy, in conjunction with both stains, constitutes a useful tool for the evaluation of apoptotic DNA fragmentation.
为了简单直接地评估抗Fas抗体诱导的小鼠培养肝细胞凋亡过程中的DNA片段化，我们通过光激发荧光显微镜检查了用溴化乙锭和4′-6-二脒基-2-苯基吲哚染色的细胞核中的荧光强度。与非凋亡细胞相比，凋亡细胞的核DNA的前一种染色强度明显降低，而凋亡组和非凋亡组之间后一种染色的荧光强度没有差异。因此，结合这两种染色剂使用光激发荧光显微镜，是评估凋亡DNA片段化的一种有用工具。
