Singh A, Shukla Y
Laboratory of Environmental Carcinogenesis, Industrial Toxicology Research Centre, Lucknow, India.
Biomed Environ Sci. 1998 Sep;11(3):258-63.
It has been reported that diallyl sulfide (DAS), a sulfur-containing volatile compound in garlic (Allium sativum), exerts anticarcinogenic activity in various rodent tumor models. In the present study, the antitumor property of DAS was tested in Swiss albino mice in the two stage initiation-promotion mouse skin carcinogenesis. Skin cancers were initiated topically with a single subcarcinogenic dose (52 micrograms) of 7, 12-dimethyl benz (a) anthracene (DMBA). Promotion was performed by twice weekly applications of 12-O-tetradecanoyl phorbol-13-acetate (TPA) at a dose of 5 micrograms/animal for 32 weeks. DAS was applied topically (250 micrograms/animal) thrice weekly for 3 weeks for anti-initiating and 1 h prior to each promotion treatment for anti-promoting studies. The results showed that the treatment schedule of DAS can effectively delay the onset of tumorigenesis and reduce the cumulative number of tumors and the average number of tumors per mouse. In groups in which DAS applied prior to initiation or promotion, a significant population of the animals remained tumor-free till the termination of experiment. These findings suggest that DAS can effectively inhibit chemically induced mouse skin carcinogenesis.
据报道，二烯丙基硫醚（DAS）是大蒜（葱属植物）中的一种含硫挥发性化合物，在各种啮齿动物肿瘤模型中具有抗癌活性。在本研究中，在瑞士白化小鼠的两阶段启动-促进小鼠皮肤癌发生模型中测试了DAS的抗肿瘤特性。皮肤癌通过单次亚致癌剂量（52微克）的7,12-二甲基苯并（a）蒽（DMBA）局部启动。促进过程通过每周两次应用剂量为5微克/动物的12-O-十四烷酰佛波醇-13-乙酸酯（TPA）进行，持续32周。DAS以250微克/动物的剂量每周三次局部应用3周用于抗启动研究，并在每次促进治疗前1小时用于抗促进研究。结果表明，DAS的治疗方案可以有效地延迟肿瘤发生的起始，并减少每只小鼠的肿瘤累积数量和平均肿瘤数量。在启动或促进之前应用DAS的组中，相当数量的动物直到实验结束都没有肿瘤。这些发现表明，DAS可以有效地抑制化学诱导的小鼠皮肤癌发生。
