Bialek R, Bartmann P
Institut für Tropenmedizin des Universitätsklinikums Tübingen, Germany.
Infection. 1998 Nov-Dec;26(6):375-8. doi: 10.1007/BF02770839.
The percentage of neutrophils phagocytosing group B streptococci (GBS) in vitro was determined in ten healthy preterm infants (< 32 weeks of gestation) and adult controls by using an acridine orange fluorescence whole blood assay. When GBS were opsonized with adult serum, no difference in phagocytic activity was found between both groups after 10 and 30 min (preterms: 40% and 68%, adults: 32% and 56%, respectively). Phagocytosis rates in preterm infants decreased significantly to 6% and 18% (at 10 and 30 min) when pool serum of preterm infants was used instead. Supplementation of the preterm serum with either intravenous immunoglobulin or IgM-enriched immunoglobulin did not change the results significantly. The addition of granulocyte colony-stimulating factor (G-CSF) accelerated phagocytosis significantly after 10 min, but did not increase the overall phagocytic activity after 30 min in either group. Hence the potential benefits of intravenous immunoglobulins and G-CSF in neonatal sepsis may not be attributable to an immediate increase in and direct effect on neutrophil phagocytic activity.
通过吖啶橙荧光全血检测法，测定了10名健康早产儿（妊娠<32周）和成年对照组体外吞噬B族链球菌（GBS）的中性粒细胞百分比。当GBS用成人血清调理后，两组在10分钟和30分钟后的吞噬活性无差异（早产儿分别为40%和68%，成年人分别为32%和56%）。而改用早产儿混合血清时，早产儿的吞噬率在10分钟和30分钟时显著降至6%和18%。给早产儿血清补充静脉注射免疫球蛋白或富含IgM的免疫球蛋白，结果无显著变化。添加粒细胞集落刺激因子（G-CSF）在10分钟后显著加速了吞噬作用，但两组在30分钟后总体吞噬活性均未增加。因此，静脉注射免疫球蛋白和G-CSF在新生儿败血症中的潜在益处可能并非归因于中性粒细胞吞噬活性的立即增加和直接作用。
