Cimato A, Facorro G, Aguirre F, Hager A, De Paoli T, Ihlo J, Farach H A, Poole C P
Cátedra de Física, Facultad de Farmacia y Bioquímica and LANAIS-RLBM, Universidad de Buenos Aires, Argentina.
Spectrochim Acta A Mol Biomol Spectrosc. 1998 Oct;54A(12):2001-8. doi: 10.1016/s1386-1425(98)00154-1.
A modification of the Asakawa-Matsushita iodometric assay method for the determination of the content of lipid hydroperoxides was developed which permits the simultaneous processing of many samples of high lipid content. The method has the advantages of simplicity as well as good reproducibility, so it is not necessary to process standards with each determination. Our technique exceeds the sensitivity attained with other spectrophotometric determinations reported in the literature. The method requires the total elimination of water from the samples, and this was accomplished using an azeotropic mixture of ethanol:water of 96:4. The results obtained with liposomes indicate that the method is applicable to biological material limited to small volume samples, ranging 5-50 microliters. We want to emphasize that this method permits the study of the peroxidation process as function of time.
开发了一种对浅川-松下碘量法的改进方法，用于测定脂质氢过氧化物的含量，该方法允许同时处理许多高脂质含量的样品。该方法具有操作简单和重现性好的优点，因此每次测定无需处理标准品。我们的技术超过了文献中报道的其他分光光度法测定所达到的灵敏度。该方法要求从样品中完全除去水分，这是通过使用96:4的乙醇:水共沸混合物来实现的。用脂质体获得的结果表明，该方法适用于体积有限的生物材料小体积样品，范围为5-50微升。我们想强调的是，该方法允许研究过氧化过程随时间的变化。
