Rosenblat G, Perelman N, Katzir E, Gal-Or S, Jonas A, Nimni M E, Sorgente N, Neeman I
Department of Food Engineering and Biotechnology, Technion-Israel Institute of Technology, Haifa.
Connect Tissue Res. 1998;37(3-4):303-11. doi: 10.3109/03008209809002447.
Acylated derivatives of ascorbic acid were found to be active in a number of biochemical and physiological processes. In the present study we investigated the effects of 6-O-palmitoyl ascorbate on collagen synthesis by cultured foreskin human fibroblasts. Our observations indicate a marked stimulatory effect on collagen synthesis by 6-O-palmitoyl ascorbate in the concentration range of 5-20 microM, while the synthesis stimulated by ascorbic acid was maximal at concentrations of 20-100 microM. Cells treated with 10 microM palmitoyl ascorbate for 36 h exhibited a production of collagen threefold greater than those in the presence of 10 microM ascorbic acid, and it was about the same as in cells treated with 100 microM ascorbic acid. By 48 h differences were not significant. Acylated ascorbate impaired vitality of the treated fibroblasts at concentrations exceeding 20 microM in media supplemented with 0.5% FCS. However, most of the cytotoxic effect was neutralized by FCS at a concentration of 10%. The resistance of acylated ascorbate against oxidative degradation as well as the role of free radicals in the modulation of collagen synthesis by ascorbic acid and by its derivatives is discussed.
已发现抗坏血酸的酰化衍生物在许多生化和生理过程中具有活性。在本研究中，我们研究了6-O-棕榈酰抗坏血酸对培养的人包皮成纤维细胞胶原蛋白合成的影响。我们的观察结果表明，在5-20微摩尔浓度范围内，6-O-棕榈酰抗坏血酸对胶原蛋白合成有显著的刺激作用，而抗坏血酸刺激的合成在20-100微摩尔浓度时达到最大值。用10微摩尔棕榈酰抗坏血酸处理36小时的细胞，其胶原蛋白产量比用10微摩尔抗坏血酸处理的细胞高三倍，与用100微摩尔抗坏血酸处理的细胞大致相同。到48小时时，差异不显著。在补充有0.5%胎牛血清的培养基中，酰化抗坏血酸在浓度超过20微摩尔时会损害处理过的成纤维细胞的活力。然而，10%浓度的胎牛血清可中和大部分细胞毒性作用。本文还讨论了酰化抗坏血酸对氧化降解的抗性以及自由基在抗坏血酸及其衍生物调节胶原蛋白合成中的作用。
