Murad S, Tajima S, Johnson G R, Sivarajah S, Pinnell S R
J Invest Dermatol. 1983 Aug;81(2):158-62. doi: 10.1111/1523-1747.ep12543573.
In confluent human skin fibroblasts maintained in 0.5% serum-supplemented medium. L-ascorbate specifically stimulated the rate of incorporation of labeled proline into total collagenase-sensitive protein, without changing the specific activity of the intracellular free proline. This influence of ascorbate reached a maximum at 30 microM and continued for at least 4 days, resulting in a 4-fold increase. The ascorbate effect occurred in cells at both confluent and subconfluent densities and was evident at all serum concentrations from 0.5-20%. The effect was independent of duration of the radioactive pulse between 2-6 h. D-Ascorbate, D-isoascorbate, and L-dehydroascorbate also stimulated collagen synthesis but at considerably higher concentrations, i.e., 250-300 microM. The stimulation of collagen synthesis by ascorbate and its analogs was accompanied by a decline in prolyl hydroxylase activity and a rise in lysyl hydroxylase activity; again L-ascorbate was found to be most effective. Dimethyltetrahydropterine and L-lactate failed to produce these effects.
在添加0.5%血清的培养基中培养的汇合的人皮肤成纤维细胞中。L-抗坏血酸特异性刺激标记脯氨酸掺入总胶原酶敏感蛋白的速率，而不改变细胞内游离脯氨酸的比活性。抗坏血酸的这种影响在30 microM时达到最大值，并持续至少4天，导致增加4倍。抗坏血酸的作用在汇合和亚汇合密度的细胞中均出现，并且在0.5-20%的所有血清浓度下均明显。该作用与2-6小时之间放射性脉冲的持续时间无关。D-抗坏血酸、D-异抗坏血酸和L-脱氢抗坏血酸也刺激胶原蛋白合成，但浓度要高得多，即250-300 microM。抗坏血酸及其类似物对胶原蛋白合成的刺激伴随着脯氨酰羟化酶活性的下降和赖氨酰羟化酶活性的上升；同样发现L-抗坏血酸最有效。二甲基四氢蝶呤和L-乳酸未能产生这些作用。
