Kudo N, Bandai N, Kawashima Y
Faculty of Pharmaceutical Sciences, Josai University, Sakado, Saitama, Japan.
Toxicol Lett. 1998 Nov 12;99(3):183-90. doi: 10.1016/s0378-4274(98)00154-4.
A method for the determination of tissue perfluorocarboxylic acids (PFCA) was developed using gas-liquid chromatography with an electron capture detector (ECD). Perfluorooctanoic acid (PFOA), perfluorononanoic acid (PFNA), and perfluorodecanoic acid (PFDA) were efficiently extracted from rat liver, methylated with diazomethane, and separated on GLC. Internal standards that were added to liver homogenates were used in the quantitative analysis of PFCAs to correct the loss during the extraction and derivatization. The concentration of PFDA in rat liver 24 h after intraperitoneal administration at a dose of 20 mg/kg body weight was 113.9 +/- 11.4 microg/g liver. The value corresponds to the previously reported results that were obtained using [14C]PFDA.
建立了一种采用带电子捕获检测器（ECD）的气液色谱法测定组织中全氟羧酸（PFCA）的方法。全氟辛酸（PFOA）、全氟壬酸（PFNA）和全氟癸酸（PFDA）能从大鼠肝脏中有效提取出来，用重氮甲烷进行甲基化，然后在气相色谱仪上分离。添加到肝脏匀浆中的内标用于全氟羧酸的定量分析，以校正提取和衍生过程中的损失。以20mg/kg体重的剂量腹腔注射后24小时，大鼠肝脏中PFDA的浓度为113.9±11.4μg/g肝脏。该值与先前使用[14C]PFDA获得的报道结果相符。
