芦丁对大肠杆菌AB1157存活及质粒PUC 9.1 DNA电泳迁移率的生物学效应。

Biological effects of rutin on the survival of Escherichia coli AB1157 and on the electrophoretic mobility of plasmid PUC 9.1 DNA.

作者信息

Bernardo Luciana C, de Oliveira Márcia B N, da Silva Cláudia R, Dantas Flávio J S, de Matros José C P, Caldeira-de-Araújo Adriano, Moura Roberto S, Bernardo-Filho Mario

机构信息

Universidade do Estado do Rio de Janeiro, Instituto de Biologia Roberto Alcantara Gomes, Departamento de Biofisica e Biometria, RJ, Brazil.

出版信息

Cell Mol Biol (Noisy-le-grand). 2002 Jul;48(5):517-20.

PMID:12146706

Abstract

The use of natural products as medicines is growing in the world. The rutin, a compound isolated from Ruta graveolens, is a flavonoid, which has been suggested to have antioxidant properties and to reduce the triacylglycerol levels. In this study, plasmid desoxyribonucleic acid (DNA) was exposed to rutin (0.33, 10, 20, 30 microg/ml) in presence of stannous chloride (SnCl2), a reducing agent widely used to obtain radiopharmaceuticals labeled with technetium-99m. Samples of the plasmid DNA were analyzed through agarose gel electrophoresis. E. coli AB1157 culture was also incubated with rutin (3, 30, 50, 100 microg/ml) and the survival fractions were calculated. The results show that the rutin, in these concentrations, is not capable of: i/ damaging the DNA, ii/ protecting the DNA from the SnCl2 redox action, and iii/ inactivating the E. coli AB1157 culture. The analysis of our data indicates that rutin do not present toxic activity in the evaluated systems.

摘要

在世界范围内，将天然产物用作药物的情况正在增加。芦丁是从芸香中分离出的一种化合物，属于黄酮类，有人认为它具有抗氧化特性并能降低三酰甘油水平。在本研究中，质粒脱氧核糖核酸（DNA）在氯化亚锡（SnCl₂）存在的情况下暴露于芦丁（0.33、10、20、30微克/毫升）中，氯化亚锡是一种广泛用于获取用锝-99m标记的放射性药物的还原剂。通过琼脂糖凝胶电泳对质粒DNA样本进行分析。大肠杆菌AB1157培养物也与芦丁（3、30、50、100微克/毫升）一起孵育，并计算存活分数。结果表明，在这些浓度下，芦丁不能：i/ 损伤DNA，ii/ 保护DNA免受SnCl₂的氧化还原作用，以及iii/ 使大肠杆菌AB1157培养物失活。对我们数据的分析表明，芦丁在评估系统中不具有毒性活性。