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两种烟草UAG抑制性tRNA(Gln)同工受体及其基因的核苷酸序列与功能特性

Nucleotide sequences and functional characterization of two tobacco UAG suppressor tRNA(Gln) isoacceptors and their genes.

作者信息

Grimm M, Nass A, Schüll C, Beier H

机构信息

Institut für Biochemie, Bayerische Julius-Maximilians-Universität, Biozentrum, Würzburg, Germany.

出版信息

Plant Mol Biol. 1998 Nov;38(5):689-97. doi: 10.1023/a:1006068303683.

DOI:10.1023/a:1006068303683
PMID:9862487
Abstract

We isolated and sequenced the two major tRNA(Gln) isoacceptors with CUG and UmUG anticodons from the cytoplasm of Nicotiana rustica. These are the first tRNAs(Gln) of nuclear origin characterized in plants. The tRNA(Gln) sequences were used to design probes for the isolation of the corresponding genes from a nuclear DNA library of N. rustica. The two cloned Nicotiana tRNA(Gln) genes, coding for either of the two isoacceptors, are efficiently transcribed in HeLa cell nuclear extract. In vitro translation in the presence of purified Nicotiana tRNAs(Gln) was carried out in a wheat germ extract partially depleted of endogenous tRNAs. Cytoplasmic (cyt) tRNA(Gln)CUG and to a lesser extent cyt tRNA(Gln)UmUG stimulated readthrough over the UAG stop codon present in the tobacco mosaic virus-specific context. The two tRNA(Gln) isoacceptors are the second class of natural UAG suppressors identified in plants, in addition to cyt tRNA(Tyr)GpsiA which has previously been characterized as the first natural UAG suppressor.

摘要

我们从黄花烟草的细胞质中分离并测序了两种主要的带有CUG和UmUG反密码子的谷氨酰胺tRNA同工受体。这些是植物中首次鉴定出的核起源的谷氨酰胺tRNA。谷氨酰胺tRNA序列被用于设计探针，以便从黄花烟草的核DNA文库中分离相应的基因。两个克隆的烟草谷氨酰胺tRNA基因，分别编码两种同工受体中的一种，在HeLa细胞核提取物中能高效转录。在部分去除了内源性tRNA的小麦胚芽提取物中，加入纯化的烟草谷氨酰胺tRNA进行体外翻译。细胞质（cyt）谷氨酰胺tRNA CUG以及程度稍低的细胞质谷氨酰胺tRNA UmUG，能在烟草花叶病毒特异性背景下，刺激越过UAG终止密码子的通读。除了之前被鉴定为第一个天然UAG抑制子的细胞质酪氨酸tRNA GpsiA外，这两种谷氨酰胺tRNA同工受体是在植物中鉴定出的第二类天然UAG抑制子。

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Plant Mol Biol. 1998 Nov;38(5):689-97. doi: 10.1023/a:1006068303683.
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本文引用的文献

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Preparation of a tRNA-dependent wheat germ protein-synthesizing system.制备 tRNA 依赖的小麦胚芽蛋白质合成体系。
Plant Mol Biol. 1989 Mar;12(3):301-6. doi: 10.1007/BF00043207.
2
Dramatic events in ciliate evolution: alteration of UAA and UAG termination codons to glutamine codons due to anticodon mutations in two Tetrahymena tRNAs.纤毛生物进化中的戏剧性事件：由于两种四膜虫 tRNA 的反密码子突变，UAA 和 UAG 终止密码子改变为谷氨酰胺密码子。
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The molecular basis for the differential translation of TMV RNA in tobacco protoplasts and wheat germ extracts.
开放阅读框 5（ORF5）中的茎环结构对于跨越翻译衣壳蛋白 ORF 终止密码子上游 700 个碱基至关重要。
J Virol. 2018 May 14;92(11). doi: 10.1128/JVI.01544-17. Print 2018 Jun 1.
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Mitochondrial genome evolution in species belonging to the Phialocephala fortinii s.l. - Acephala applanata species complex.属 Phialocephala fortinii s.l.-Acephala applanata 种复合体物种的线粒体基因组进化。
BMC Genomics. 2012 May 4;13:166. doi: 10.1186/1471-2164-13-166.
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Versatile dual reporter gene systems for investigating stop codon readthrough in plants.用于研究植物中终止密码子通读的多功能双报告基因系统。
PLoS One. 2009 Oct 9;4(10):e7354. doi: 10.1371/journal.pone.0007354.
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Misreading of termination codons in eukaryotes by natural nonsense suppressor tRNAs.真核生物中天然无义抑制tRNA对终止密码子的误读。
Nucleic Acids Res. 2001 Dec 1;29(23):4767-82. doi: 10.1093/nar/29.23.4767.
7
The trimethylamine methyltransferase gene and multiple dimethylamine methyltransferase genes of Methanosarcina barkeri contain in-frame and read-through amber codons.巴氏甲烷八叠球菌的三甲胺甲基转移酶基因和多个二甲胺甲基转移酶基因含有框内和通读琥珀密码子。
J Bacteriol. 2000 May;182(9):2520-9. doi: 10.1128/JB.182.9.2520-2529.2000.
TMV RNA 在烟草原生质体和小麦胚芽提取物中差异翻译的分子基础。
EMBO J. 1984 May;3(5):1091-6. doi: 10.1002/j.1460-2075.1984.tb01934.x.
4
UAG readthrough during TMV RNA translation: isolation and sequence of two tRNAs with suppressor activity from tobacco plants.TMV RNA 翻译中的 UAG 通读：从烟草植物中分离和测序两种具有抑制活性的 tRNA。
EMBO J. 1984 Feb;3(2):351-6. doi: 10.1002/j.1460-2075.1984.tb01810.x.
5
Wheat cytoplasmic arginine tRNA isoacceptor with a U*CG anticodon is an efficient UGA suppressor in vitro.带有U*CG反密码子的小麦细胞质精氨酸tRNA同工受体在体外是一种有效的UGA抑制子。
Nucleic Acids Res. 1998 Mar 15;26(6):1390-5. doi: 10.1093/nar/26.6.1390.
6
Nonsense suppression in mammalian cells.哺乳动物细胞中的无义抑制。
Biochimie. 1996;78(11-12):1007-15. doi: 10.1016/s0300-9084(97)86724-7.
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Nucleotide sequences of nuclear tRNA(Cys) genes from Nicotiana and Arabidopsis and expression in HeLa cell extract.烟草和拟南芥细胞核tRNA（半胱氨酸）基因的核苷酸序列及其在HeLa细胞提取物中的表达。
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8
UGA suppression by tRNACmCATrp occurs in diverse virus RNAs due to a limited influence of the codon context.由于密码子上下文的影响有限，tRNACmCATrp对UGA的抑制作用存在于多种病毒RNA中。
Nucleic Acids Res. 1996 Sep 1;24(17):3424-30. doi: 10.1093/nar/24.17.3424.
9
Molecular cloning and sequencing of a nuclear gene encoding tRNA(Gln) (UUG) from Arabidopsis thaliana.拟南芥中编码tRNA（Gln）（UUG）的一个核基因的分子克隆与测序
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The tRNATyr multigene family of Triticum aestivum: genome organization, sequence analyses and maturation of intron-containing pre-tRNAs in wheat germ extract.普通小麦的tRNATyr多基因家族：基因组组织、序列分析以及小麦胚芽提取物中含内含子前体tRNA的成熟
FEBS Lett. 1996 Apr 22;384(3):222-6. doi: 10.1016/0014-5793(96)00313-4.