• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

普通小麦的tRNATyr多基因家族：基因组组织、序列分析以及小麦胚芽提取物中含内含子前体tRNA的成熟

The tRNATyr multigene family of Triticum aestivum: genome organization, sequence analyses and maturation of intron-containing pre-tRNAs in wheat germ extract.

作者信息

Arends S, Kraus J, Beier H

机构信息

Institut für Biochemie, Bayerishche Julius-Maximillans-Universität, Biozentrum, Würzburg, Germany.

出版信息

FEBS Lett. 1996 Apr 22;384(3):222-6. doi: 10.1016/0014-5793(96)00313-4.

DOI:10.1016/0014-5793(96)00313-4
PMID:8617358
Abstract

Southern analysis of Triticum DNA has revealed that nuclear tRNATyr genes are dispersed at a minimum of 16 loci in the genome. We have isolated six independent tRNATyr genes from a Triticum aestivum library in addition to three known members of the Triticum tRNATyr family. Four of the sequenced tRNATyr genes code for Triticum tRNA Tyr and two code for tRNA2Tyr. Three genes encode tRNAsTyr which carry one or two nucleotide substitutions as compared to the conventional genes. The nine Triticum tRNATyr genes possess highly conserved intron sequences ranging in size from 12 to 14 nucleotides. A common secondary intron structure with the 5' and 3' splice site loops separated by five base pairs can be formed by all pre-tRNAs Tyr which are efficiently spliced in the homologous wheat germ extract.

摘要

对小麦DNA的Southern分析表明，核tRNATyr基因在基因组中至少分散于16个位点。除了小麦tRNATyr家族的三个已知成员外，我们还从小麦文库中分离出了六个独立的tRNATyr基因。测序的tRNATyr基因中有四个编码小麦tRNATyr，两个编码tRNA2Tyr。与传统基因相比，三个基因编码的tRNAsTyr携带一个或两个核苷酸替换。九个小麦tRNATyr基因具有高度保守的内含子序列，大小在12至14个核苷酸之间。所有能在同源小麦胚提取物中有效剪接的前体tRNAs Tyr都能形成一种常见的二级内含子结构，其5'和3'剪接位点环由五个碱基对隔开。

相似文献

1
The tRNATyr multigene family of Triticum aestivum: genome organization, sequence analyses and maturation of intron-containing pre-tRNAs in wheat germ extract.普通小麦的tRNATyr多基因家族：基因组组织、序列分析以及小麦胚芽提取物中含内含子前体tRNA的成熟
FEBS Lett. 1996 Apr 22;384(3):222-6. doi: 10.1016/0014-5793(96)00313-4.
2
The tRNATyr-isoacceptors and their genes in the ciliate Tetrahymena thermophila: cytoplasmic tRNATyr has a QPsiA anticodon and is coded by multiple intron-containing genes.嗜热四膜虫中tRNATyr同工受体及其基因：细胞质tRNATyr具有QPsiA反密码子，由多个含内含子的基因编码。
Nucleic Acids Res. 1997 Nov 1;25(21):4194-200. doi: 10.1093/nar/25.21.4194.
3
Two intron-containing pre-tRNAs(Tyr) from Triticum aestivum are efficiently processed and spliced in homologous cell-free extract.来自普通小麦的两个含内含子的前体tRNA（Tyr）在同源无细胞提取物中能被有效加工和剪接。
Acta Biochim Pol. 1992;39(3):283-8.
4
Expression of nuclear tRNA(Tyr) genes from Arabidopsis thaliana in HeLa cell and wheat germ extracts.拟南芥核tRNA（Tyr）基因在HeLa细胞和小麦胚芽提取物中的表达
Plant Mol Biol. 1991 May;16(5):865-75. doi: 10.1007/BF00015078.
5
The tRNA(Tyr) multigene family of Nicotiana rustica: genome organization, sequence analyses and expression in vitro.黄花烟草的tRNA（酪氨酸）多基因家族：基因组组织、序列分析及体外表达
Plant Mol Biol. 1992 Dec;20(5):869-78. doi: 10.1007/BF00027158.
6
Splicing of arabidopsis tRNA(Met) precursors in tobacco cell and wheat germ extracts.拟南芥tRNA（Met）前体在烟草细胞和小麦胚芽提取物中的剪接。
Plant Mol Biol. 2000 Sep;44(2):155-65. doi: 10.1023/a:1006486315360.
7
Intron excision from tRNA precursors by plant splicing endonuclease requires unique features of the mature tRNA domain.植物剪接内切核酸酶从tRNA前体中切除内含子需要成熟tRNA结构域的独特特征。
Eur J Biochem. 1992 Nov 15;210(1):193-203. doi: 10.1111/j.1432-1033.1992.tb17408.x.
8
A cell-free plant extract for accurate pre-tRNA processing, splicing and modification.一种用于精确进行前体tRNA加工、剪接和修饰的无细胞植物提取物。
EMBO J. 1987 Sep;6(9):2811-8. doi: 10.1002/j.1460-2075.1987.tb02577.x.
9
A human and a plant intron-containing tRNATyr gene are both transcribed in a HeLa cell extract but spliced along different pathways.一个人类和一个含有内含子的植物tRNATyr基因在HeLa细胞提取物中均被转录，但沿着不同的途径进行剪接。
EMBO J. 1987 Jan;6(1):35-41. doi: 10.1002/j.1460-2075.1987.tb04715.x.
10
Plant nonsense suppressor tRNA(Tyr) genes are expressed at very low levels in vitro due to inefficient splicing of the intron-containing pre-tRNAs.植物无义抑制tRNA（Tyr）基因在体外表达水平极低，原因是含内含子的前体tRNA剪接效率低下。
Nucleic Acids Res. 1991 Feb 25;19(4):707-12. doi: 10.1093/nar/19.4.707.

引用本文的文献

1
Plant tRNA ligases are multifunctional enzymes that have diverged in sequence and substrate specificity from RNA ligases of other phylogenetic origins.植物tRNA连接酶是多功能酶，其在序列和底物特异性上与其他系统发育起源的RNA连接酶有所不同。
Nucleic Acids Res. 2005 Jan 14;33(1):388-99. doi: 10.1093/nar/gki174. Print 2005.
2
Translational nonsense codon suppression as indicator for functional pre-tRNA splicing in transformed Arabidopsis hypocotyl-derived calli.翻译无义密码子抑制作为转化的拟南芥下胚轴来源愈伤组织中功能性前体tRNA剪接的指标。
Nucleic Acids Res. 2003 Feb 15;31(4):1197-207. doi: 10.1093/nar/gkg220.
3
Splicing of arabidopsis tRNA(Met) precursors in tobacco cell and wheat germ extracts.
拟南芥tRNA（Met）前体在烟草细胞和小麦胚芽提取物中的剪接。
Plant Mol Biol. 2000 Sep;44(2):155-65. doi: 10.1023/a:1006486315360.
4
Nucleotide sequences and functional characterization of two tobacco UAG suppressor tRNA(Gln) isoacceptors and their genes.两种烟草UAG抑制性tRNA(Gln)同工受体及其基因的核苷酸序列与功能特性
Plant Mol Biol. 1998 Nov;38(5):689-97. doi: 10.1023/a:1006068303683.
5
Structure and expression of several bean (Phaseolus vulgaris) nuclear transfer RNA genes: relevance to the process of tRNA import into plant mitochondria.几种菜豆（Phaseolus vulgaris）核转运RNA基因的结构与表达：与tRNA导入植物线粒体过程的相关性
Plant Mol Biol. 1998 Mar;36(4):613-25. doi: 10.1023/a:1005972023506.
6
Nucleotide sequences of nuclear tRNA(Cys) genes from Nicotiana and Arabidopsis and expression in HeLa cell extract.烟草和拟南芥细胞核tRNA（半胱氨酸）基因的核苷酸序列及其在HeLa细胞提取物中的表达。
Plant Mol Biol. 1996 Nov;32(3):549-52. doi: 10.1007/BF00019108.
7
Another heritage from the RNA world: self-excision of intron sequence from nuclear pre-tRNAs.RNA世界的另一个遗产：从细胞核前体tRNA中自我切除内含子序列。
Nucleic Acids Res. 1996 Jun 15;24(12):2212-9. doi: 10.1093/nar/24.12.2212.