Hardin J A, Chung B, O'loughlin E V, Gall D G
Gastrointestinal Research Group, Health Sciences Centre, University of Calgary, Calgary, Alberta, Canada.
Gut. 1999 Jan;44(1):26-32. doi: 10.1136/gut.44.1.26.
Epidermal growth factor (EGF) has been shown to increase intestinal absorptive surface area and transport function in normal animals.
To examine the effect of EGF on absorptive surface area and brush border membrane function in a model of massive small bowel resection.
New Zealand white rabbits were randomised into two groups: a resected group (60% proximal small bowel resection); and an unmanipulated control group. Distal remnant tissue was examined 10 and 21 days postsurgery. In separate experiments oral EGF (40 g/kg/day) was administered to resected animals from days 3 to 8 and animals were studied on day 10.
Ten days postsurgery brush border surface area and total absorptive surface area were significantly increased in remnant tissue while brush border membrane vesicle (BBMV) glucose uptake was significantly decreased compared with controls. By 21 days brush border surface area returned to control levels though BBMV glucose uptake remained depressed. EGF treatment induced a further increase in brush border surface area in remnant intestine but did not alter BBMV glucose uptake.
Surgical resection results in significant elevations in absorptive surface area coupled with a decrease in brush border membrane transport function distal to the site of anastomosis. EGF enhances glucose uptake in remnant intestine via recruitment of additional microvillus membrane into the brush border.
表皮生长因子（EGF）已被证明可增加正常动物的肠道吸收表面积和转运功能。
在大规模小肠切除模型中研究EGF对吸收表面积和刷状缘膜功能的影响。
将新西兰白兔随机分为两组：切除组（近端小肠切除60%）；和未处理的对照组。在术后10天和21天检查远端残余组织。在单独的实验中，从第3天到第8天对切除动物给予口服EGF（40μg/kg/天），并在第10天对动物进行研究。
术后10天，残余组织中的刷状缘表面积和总吸收表面积显著增加，而与对照组相比，刷状缘膜囊泡（BBMV）葡萄糖摄取显著降低。到21天时，刷状缘表面积恢复到对照水平，尽管BBMV葡萄糖摄取仍处于抑制状态。EGF治疗导致残余小肠刷状缘表面积进一步增加，但未改变BBMV葡萄糖摄取。
手术切除导致吻合口远端的吸收表面积显著增加，同时刷状缘膜转运功能降低。EGF通过将额外的微绒毛膜募集到刷状缘来增强残余小肠中的葡萄糖摄取。
