O'Loughlin E, Winter M, Shun A, Hardin J A, Gall D G
Department of Paediatrics, John Hunter Hospital, New Lambton, New South Wales, Australia.
Gastroenterology. 1994 Jul;107(1):87-93. doi: 10.1016/0016-5085(94)90064-7.
BACKGROUND/AIMS: Remnant small intestine undergoes adaptation following massive resection. The aim of this study was to examine the effect of epidermal growth factor (EGF) on ileal adaptation following proximal resection.
New Zealand white rabbits, aged 8 weeks, underwent 2/3 proximal resection, and ileal mucosal adaptation was assessed 10 and 21 days postsurgery. In a second series of experiments, animals with resection received oral EGF (40 micrograms.kg-1.day-1) for 5 days, and the effect on adaptation was assessed 10 days postsurgery.
Transection alone stimulated mucosal hyperplasia, intestinal sucrase specific activity, and glucose transport at 10 days but not at 21 days. Resection resulted in mucosal hyperplasia at both time periods and increased disaccharidase specific activity at 10 days postresection. In contrast, 3 O-methyl-D-glucose transport was significantly decreased compared with both groups at both time periods. EGF treatment in animals with resection did not alter mucosal proliferation but did stimulate maltase specific activity and caused a 3-4-fold increase in glucose transport and phlorizin binding.
Following proximal resection, adaptation of intestinal digestive and absorptive function does not parallel mucosal hyperplasia. Administration of EGF to resected animals enhances glucose absorption and may have a therapeutic role in the management of short gut syndrome.
背景/目的：大量小肠切除术后，残余小肠会发生适应性变化。本研究旨在探讨表皮生长因子（EGF）对近端小肠切除术后回肠适应性的影响。
选取8周龄的新西兰白兔，进行2/3近端小肠切除，术后10天和21天评估回肠黏膜适应性。在第二组实验中，切除术后的动物口服EGF（40微克·千克-1·天-1），持续5天，术后10天评估其对适应性的影响。
单纯横断术在术后10天可刺激黏膜增生、提高肠蔗糖酶比活性和葡萄糖转运，但在术后21天无此作用。切除术后两个时间段均出现黏膜增生，且术后10天双糖酶比活性增加。相比之下，两个时间段3-O-甲基-D-葡萄糖转运在两组中均显著降低。切除术后动物接受EGF治疗未改变黏膜增殖，但刺激了麦芽糖酶比活性，并使葡萄糖转运和根皮苷结合增加3至4倍。
近端小肠切除术后，肠道消化和吸收功能的适应性变化与黏膜增生并不平行。给切除术后的动物施用EGF可增强葡萄糖吸收，可能在短肠综合征的治疗中发挥作用。
