Wang H, Hartswood E, Finnegan D J
Institute of Cell and Molecular Biology, University of Edinburgh, King's Buildings, Edinburgh EH9 3JR, UK.
Nucleic Acids Res. 1999 Jan 15;27(2):455-61. doi: 10.1093/nar/27.2.455.
Pogo is a transposable element with short terminal inverted repeats. It contains two open reading frames that are joined by splicing and code for the putative pogo transposase, the sequence of which indicates that it is related to the transposases of members of the Tc1/mariner family as well as proteins that have no known transposase activity including the centromere binding protein CENP-B. We have shown that the N-terminal region of pogo transposase binds in a sequence-specific manner to the ends of pogo and have identified residues essential for this. The results are consistent with a prediction that DNA binding is due to a helix-turn-helix motif within this region. The transposase recognises a 12 bp sequence, two copies of which are present at each end of pogo DNA. The outer two copies occur as inverted repeats 14 nucleotides from each end of the element, and contain a single base mismatch and indicate the inverted repeats of pogo are 26 nucleotides long. The inner copies occur as direct repeats, also with a single mismatch.
Pogo是一种具有短末端反向重复序列的转座元件。它包含两个通过剪接连接的开放阅读框，编码假定的Pogo转座酶，其序列表明它与Tc1/水手家族成员的转座酶以及包括着丝粒结合蛋白CENP-B在内的无已知转座酶活性的蛋白质相关。我们已经表明，Pogo转座酶的N端区域以序列特异性方式结合到Pogo的末端，并确定了对此至关重要的残基。结果与DNA结合是由于该区域内的螺旋-转角-螺旋基序的预测一致。转座酶识别一个12 bp的序列，其两个拷贝存在于Pogo DNA的每一端。外侧的两个拷贝以反向重复的形式出现，距离元件的每一端14个核苷酸，并且包含一个单碱基错配，表明Pogo的反向重复序列长26个核苷酸。内侧的拷贝以正向重复的形式出现，也有一个单碱基错配。
