Mohanam S, Go Y, Sawaya R, Venkaiah B, Mohan P M, Kouraklis G P, Gokaslan Z L, Lagos G K, Rao J S
Department of Neurosurgery, The University of Texas M.D. Anderson Cancer Center, Houston, TX 77030, USA.
Int J Oncol. 1999 Jan;14(1):169-74. doi: 10.3892/ijo.14.1.169.
Urokinase-type plasminogen activator (uPA) and its receptor (uPAR) are important in the regulation of tumor tissue progenesis, cell differentiation, tumor cell motility, and tumor cell invasiveness. We have recently reported that the levels of uPA and uPAR were higher in malignant astrocytomas than in low-grade gliomas. In the present study, we measured the levels of uPA and uPAR during the growth of glioblastomas in nude mice. Using fibrin zymography, densitometry, and an enzyme-linked immunosorbent assay, we found that the enzyme activity and content of uPA were increased 4- to 10-fold during tumor formation. Using a receptor assay and an enzyme linked immunosorbent assay, we found the numbers and content of uPAR were increased 5- to 15-fold during tumor formation. In addition, immunohistochemical staining for uPA and uPAR revealed strong immunoreactivity in tumor cells with the staining more intense on day 28 than on day 14. These results suggest that the upregulation of uPA and uPAR plays a major role in the formation of gliomas.
尿激酶型纤溶酶原激活剂（uPA）及其受体（uPAR）在肿瘤组织发生、细胞分化、肿瘤细胞运动及肿瘤细胞侵袭的调控中起重要作用。我们最近报道，恶性星形细胞瘤中uPA和uPAR的水平高于低级别胶质瘤。在本研究中，我们测定了裸鼠成胶质细胞瘤生长过程中uPA和uPAR的水平。通过纤维蛋白酶谱法、光密度测定法及酶联免疫吸附测定，我们发现肿瘤形成过程中uPA的酶活性和含量增加了4至10倍。通过受体测定及酶联免疫吸附测定，我们发现肿瘤形成过程中uPAR的数量和含量增加了5至15倍。此外，uPA和uPAR的免疫组织化学染色显示肿瘤细胞中有强免疫反应性，且第28天的染色比第14天更强烈。这些结果表明，uPA和uPAR的上调在胶质瘤形成中起主要作用。
