Xu X G, Zhu J Q, Lu Q
Department of Biology, Suzhou Railway Teacher's College, China.
Zhongguo Yao Li Xue Bao. 1996 Sep;17(5):460-2.
To study the effect of GABA on cold restraint-induced gastric ulcer (CRGU) and its mechanism.
CRGU was produced in adult male mice at 1 degree C in cold room under restraint for 1 h. The ulcer index indicated the severity of gastric mucosa lesion. Gastric mucin was determined with alcian blue dye.
GABA 2 mumol (i.c.v.) accelerated CRGU, Bicuculline (Bic) 0.2 mumol (i.c.v.) did not affect CRGU, whereas Baclofen (Bac) 4 mumol (i.c.v.) exacerbated CRGU. Bic did not modify the exacerbation of CRGU by GABA and Bac. Atropine (Atr) 0.2 mg.kg-1 (s.c.) and Phentolamine (Phen) 2.5 mg.kg-1 inhibited CRGU, and abolished the exacerbation of CRGU by GABA. Cold restraint (CR) decreased the amount of the gastric mucin, but GABA 2 mumol had no effect on CR gastric mucin.
While GABA-B receptor in brain was activated, exogenous GABA exacerbated CRGU via vagal and sympathetic nerves, bearing no relation to decrease of the gastric mucin or weakening of the gastric mucosal barrier.
研究γ-氨基丁酸（GABA）对冷束缚应激诱导的胃溃疡（CRGU）的影响及其机制。
将成年雄性小鼠置于1℃的冷室中束缚1小时以制备CRGU。溃疡指数表明胃黏膜损伤的严重程度。用阿尔辛蓝染料测定胃黏液。
GABA 2 μmol（脑室内注射）加速了CRGU的形成，荷包牡丹碱（Bic）0.2 μmol（脑室内注射）对CRGU无影响，而巴氯芬（Bac）4 μmol（脑室内注射）加剧了CRGU的形成。Bic并未改变GABA和Bac对CRGU的加剧作用。阿托品（Atr）0.2 mg·kg-1（皮下注射）和酚妥拉明（Phen）2.5 mg·kg-1抑制了CRGU，并消除了GABA对CRGU的加剧作用。冷束缚（CR）减少了胃黏液的量，但GABA 2 μmol对CR时的胃黏液无影响。
当脑内GABA-B受体被激活时，外源性GABA通过迷走神经和交感神经加剧CRGU，这与胃黏液减少或胃黏膜屏障减弱无关。
