Pei L, Zong Y Y, Sun Y F, Zhang G Y
Research Center of Biochemistry and Molecular Biology, Xuzhou Medical College.
Yao Xue Xue Bao. 1996;31(11):812-6.
The effects of glutamate on cultured cortical neurons and the protective effect of ketamine and nifedipine were studied. On day 10 after plating of the cortical cells from 16-18 day-old fetal rats, the cultures were exposed to 50 mumol.L-1 glutamate and low glucose (1 g.L-1) for 10 min-24 h. The results showed that a release of lactate dehydrogenase (LDH) into the culture supernatant was observed as a function of time. The values of LDH efflux in culture medium was significantly lower than those of controls when the cells were pretreated with ketamine or nifedipine 10 min prior to addition of glutamate. More significant decrease of LDH activity in culture medium was observed when the two drugs were used in combination. These results demonstrate that the dissociated cultured cortical neurons from fetal rat are seriously damaged by glutamate. Such damage could be attenuated by ketamine and nifedipine, suggesting that ketamine and nifedipine may protect neurons from the glutamate toxicity, and the effect of combining ketamine and nifedipine was greater than either ketamine or nifedipine alone.
研究了谷氨酸对培养的皮层神经元的作用以及氯胺酮和硝苯地平的保护作用。从16 - 18日龄胎鼠分离的皮层细胞接种后第10天，将培养物暴露于50μmol.L-1谷氨酸和低糖（1g.L-1）环境中10分钟至24小时。结果显示，培养上清液中乳酸脱氢酶（LDH）的释放随时间变化。在添加谷氨酸前10分钟用氯胺酮或硝苯地平预处理细胞时，培养基中LDH流出值显著低于对照组。当两种药物联合使用时，培养基中LDH活性的降低更为显著。这些结果表明，胎鼠解离培养的皮层神经元受到谷氨酸的严重损伤。氯胺酮和硝苯地平可减轻这种损伤，提示氯胺酮和硝苯地平可能保护神经元免受谷氨酸毒性作用，且氯胺酮和硝苯地平联合使用的效果大于单独使用氯胺酮或硝苯地平。
