淋病奈瑟菌lbpB基因产物的鉴定与功能特性分析
Identification and functional characterization of the Neisseria gonorrhoeae lbpB gene product.
作者信息
Biswas G D, Anderson J E, Chen C J, Cornelissen C N, Sparling P F
机构信息
Department of Medicine, School of Medicine, University of North Carolina at Chapel Hill 27599, USA.
出版信息
Infect Immun. 1999 Jan;67(1):455-9. doi: 10.1128/IAI.67.1.455-459.1999.
Abstract
We cloned lbpB, encoding a predicted 80-kDa lipoprotein, upstream of lbpA. A nonpolar mutant (LbpB- LbpA+) had normal lactoferrin (LF) binding and grew normally with LF as an iron source, whereas LbpB- LbpA- and LbpB+ LbpA- strains had reduced binding of LF and did not grow with LF as an iron source. LbpB bound LF directly in an affinity purification, suggesting that LbpB might play a still-uncharacterized role in the LF iron utilization.
摘要
我们在lbpA上游克隆了编码预测分子量为80 kDa脂蛋白的lbpB。一个非极性突变体（LbpB - LbpA +）具有正常的乳铁蛋白（LF）结合能力，并且以LF作为铁源时生长正常，而LbpB - LbpA - 和LbpB + LbpA - 菌株对LF的结合减少，并且不能以LF作为铁源生长。在亲和纯化中，LbpB直接结合LF，这表明LbpB可能在LF铁利用中发挥尚未明确的作用。
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2
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3
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