一个新的枯草芽孢杆菌基因antE，受时间调控，与dnaE基因反向且重叠。
A novel Bacillus subtilis gene, antE, temporally regulated and convergent to and overlapping dnaE.
作者信息
Wang L F, Park S S, Doi R H
机构信息
Section of Molecular and Cellular Biology, University of California, Davis, California 95616, USA.
出版信息
J Bacteriol. 1999 Jan;181(1):353-6. doi: 10.1128/JB.181.1.353-356.1999.
Abstract
A Bacillus subtilis promoter, Px, that functions in a convergent manner with the sigA operon promoter P3 has been found in the sigA operon. Promoter Px is turned on at the same time as promoter P3 during early sporulation. The transcript from promoter Px codes for a small protein with partial homology to the OmpR protein from Escherichia coli and also carries an untranslated sequence at its 3' end that is complementary to the 5' end of the P3 transcript, which codes for the ribosome binding site of dnaE. The gene controlled by Px has been called antE. The expression of antE does not require sigmaB, sigmaE, or sigmaH. Px was transcribed in vitro by the sigmaA holoenzyme and is the seventh promoter to be recognized in the sigmaA operon. A possible role for the antE gene during early sporulation is proposed.
摘要
在枯草芽孢杆菌sigA操纵子中发现了一个与sigA操纵子启动子P3以汇聚方式起作用的枯草芽孢杆菌启动子Px。在早期芽孢形成过程中，启动子Px与启动子P3同时开启。来自启动子Px的转录本编码一种与大肠杆菌OmpR蛋白具有部分同源性的小蛋白，并且在其3'端还带有一个未翻译序列，该序列与P3转录本的5'端互补，P3转录本编码dnaE的核糖体结合位点。由Px控制的基因被称为antE。antE的表达不需要sigmaB、sigmaE或sigmaH。Px在体外由sigmaA全酶转录，并且是在sigmaA操纵子中被识别的第七个启动子。文中提出了antE基因在早期芽孢形成过程中的可能作用。
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