Neyfakh E A, Alimbekova A I, Ivanenko G F
Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, 117977, Russia.
Biochemistry (Mosc). 1998 Oct;63(10):1138-43.
Essential lipid bioantioxidants (BAO) (vitamins E and A) and catabolic products of the lipoperoxide cascade (LPC) (diene conjugates, ketodienes, and carbonyls) were determined in blood plasma of children aged 11 days to three years (n = 123) who are residents of regions contaminated with radionuclides (5-40 Ci/km2 of 137Cs) discharged during the Chernobyl power plant accident. Individual antenatal radiation loads Da of children and total radiation doses of their mothers Dm were also determined as external gamma-radiation loads, which correlate with internal radiation doses for permanent residents of contaminated regions. Before examination, therapy with multivitamins containing BAO determined in this work was administered to groups of pregnant women and their children (group I, n = 48) age-matched to a group that received no therapy (group II, n = 99). In group II, reduced concentrations of vitamins E and A decreased to levels indicating severe vitamin deficiency and displayed inverse correlations with Dm and Da. However, in group I these decreases and inverse correlation were only characteristic of initially normal levels of tocopherol. In group II, increases in Dm and Da caused stress-related increases in blood levels of diene conjugates and ketodienes (which was stronger than in group I with increases in Da) and, because of this, E and A vitamin deficiencies. In group I, LPC levels inversely correlated with Dm. We found nine significant correlations of radioloads of mothers with BAO and LPC pathologies of their children. The total pathogenic effectiveness of Dm in relation to LPC and BAO of children is comparable to the effectiveness of Da, which is the most hazardous radiation load. The possibility of existence of a radiation-induced protective response mobilizing BAO from depots into the blood stream is considered. BAO therapy increased the radiation resistance of children. A hypothesis was formulated that the uptake by children of their mother's radioactive Chernobyl isotopes in amounts adequate to their radiation loads is the mechanism of correlations of Dm with long-term pathogenic effects on BAO and LPC systems of children.
在切尔诺贝利核电站事故期间受放射性核素污染（137Cs含量为5 - 40居里/平方公里）地区居住的11天至3岁儿童（n = 123）的血浆中，测定了必需脂质生物抗氧化剂（BAO）（维生素E和A）以及脂质过氧化物级联反应（LPC）的分解产物（二烯共轭物、酮二烯和羰基化合物）。还测定了儿童个体的产前辐射剂量Da及其母亲的总辐射剂量Dm，作为外部γ辐射剂量，其与受污染地区常住居民的体内辐射剂量相关。在检查前，给与本研究中测定的含BAO的多种维生素进行治疗的孕妇及其儿童组（I组，n = 48），其年龄与未接受治疗的组（II组，n = 99）相匹配。在II组中，维生素E和A的浓度降低至表明严重维生素缺乏的水平，并与Dm和Da呈负相关。然而，在I组中，这些降低和负相关仅在生育三烯酚初始正常水平时才具有特征性。在II组中，Dm和Da的增加导致与应激相关的二烯共轭物和酮二烯血液水平升高（Da增加时比I组更明显），并因此导致E和A维生素缺乏。在I组中，LPC水平与Dm呈负相关。我们发现母亲的辐射剂量与孩子的BAO和LPC病变之间存在9个显著相关性。Dm对儿童LPC和BAO的总致病效力与最危险的辐射剂量Da的效力相当。考虑了存在辐射诱导的保护性反应，将BAO从储存库动员到血流中的可能性。BAO治疗增加了儿童的辐射抗性。提出了一个假设，即儿童摄取其母亲体内足以对应其辐射剂量的切尔诺贝利放射性同位素，是Dm与对儿童BAO和LPC系统长期致病作用之间相关性的机制。
