辛德毕斯病毒诱导的病毒核糖核酸聚合酶。
Sindbis virus-induced viral ribonucleic acid polymerase.
作者信息
Sreevalsan T, Yin F H
出版信息
J Virol. 1969 Jun;3(6):599-604. doi: 10.1128/JVI.3.6.599-604.1969.
Abstract
A cytoplasmic structure containing the viral ribonucleic acid (RNA) polymerase has been isolated by sucrose density centrifugation from cells infected with Sindbis virus. Uninfected cells did not contain any such structure. Preliminary experiments indicated that the structure may be associated with membranes. This structure incorporated (3)H-guanosine triphosphate in vitro in the absence of added template. The RNA synthesized in vitro by the enzyme consisted of single-stranded 40S RNA, the ribonuclease-resistant replicative form, and possibly the replicative intermediate form of viral RNA. The products formed in vitro by the enzyme are identical in sedimentation rates to those formed in the infected cells in vivo.
摘要
通过蔗糖密度离心法，已从感染辛德毕斯病毒的细胞中分离出一种含有病毒核糖核酸（RNA）聚合酶的细胞质结构。未感染的细胞不含任何此类结构。初步实验表明，该结构可能与膜有关。在没有添加模板的情况下，这种结构在体外能掺入（3）H-鸟苷三磷酸。该酶在体外合成的RNA由单链40S RNA、抗核糖核酸酶的复制形式以及可能的病毒RNA复制中间形式组成。该酶在体外形成的产物沉降速率与体内感染细胞中形成的产物相同。
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