Levin J G, Ramseur J M, Grimley P M
J Virol. 1973 Dec;12(6):1401-6. doi: 10.1128/JVI.12.6.1401-1406.1973.
Serial passage of Semliki Forest virus (SFV) in chicken embryo cells had little effect on SFV yield; however, high multiplicity infection of murine cells with one of the late passage pools (passage 9 SFV) resulted in a virus yield 10- to 20-fold lower than that obtained with earlier passage virus and 80-fold lower than the corresponding yield in chicken cells. This effect was accompanied by a striking decrease in the levels of 42S and 26S RNA and by increased proportions of a small single-stranded viral RNA (molecular weight, 9 x 10(5)) and of a low-molecular-weight replicative form. There was also a reduction in the number of specific membranous structures previously associated with the group A arbovirus replication complex. These results suggested that passage 9 SFV contained defective interfering particles which were detected more readily after one passage in a murine indicator host cell. Identical results were obtained with two different murine cell lines: one a leukemia virus-free clone of AKR cells and the other JLS-V9 cells chronically infected with Rauscher leukemia virus. Host production of RNA tumor virus particles apparently did not affect arbovirus replication.
在鸡胚细胞中连续传代塞姆利基森林病毒（SFV）对SFV产量影响不大；然而，用后期传代病毒库之一（第9代SFV）以高感染复数感染鼠细胞，产生的病毒产量比早期传代病毒低10至20倍，比在鸡细胞中的相应产量低80倍。这种效应伴随着42S和26S RNA水平的显著下降，以及一种小的单链病毒RNA（分子量为9×10⁵）和低分子量复制形式比例的增加。以前与A组虫媒病毒复制复合体相关的特定膜结构数量也有所减少。这些结果表明，第9代SFV含有缺陷干扰颗粒，在鼠指示宿主细胞中传代一次后更容易检测到。用两种不同的鼠细胞系获得了相同的结果：一种是无白血病病毒的AKR细胞克隆，另一种是长期感染劳斯氏白血病病毒的JLS-V9细胞。宿主产生RNA肿瘤病毒颗粒显然不影响虫媒病毒的复制。
