Ben-Yedidia T, Arnon R
Department of Immunology, The Weizmann Institute of Science, Rehovot, Israel.
Immunol Lett. 1998 Nov;64(1):9-15. doi: 10.1016/s0165-2478(98)00073-x.
In our previous studies on the development of synthetic peptide-based vaccines, we have evaluated flagellin from a Salmonella typhi vaccine strain as a carrier molecule for synthetic peptides derived from the influenza virus. The results indicated that the use of recombinant flagella, expressing defined influenza epitopes, is adequate for induction of protection against a challenge infection. It is of importance to show that previous exposure to the carrier does not induce suppression of the response to such vaccine. In the present study we demonstrate that the protective effect is not impaired by pre-immunization with either the carrier flagellin molecule alone or with the intact salmonella. The immune response was manifested both by the level of antibodies produced and by a proliferative cellular response, as well as by an efficient protection of the mice from a sub-lethal challenge infection. Since prior exposure to the carrier did not result in immune suppression, we conclude that Salmonella flagellin is a suitable carrier for synthetic peptide based vaccines.
在我们之前关于合成肽基疫苗开发的研究中，我们评估了伤寒沙门氏菌疫苗株的鞭毛蛋白作为源自流感病毒的合成肽的载体分子。结果表明，表达特定流感表位的重组鞭毛足以诱导针对攻击感染的保护作用。重要的是要表明，先前接触载体不会诱导对这种疫苗反应的抑制。在本研究中，我们证明单独用载体鞭毛蛋白分子或完整沙门氏菌进行预免疫不会损害保护作用。免疫反应通过产生的抗体水平、增殖性细胞反应以及小鼠免受亚致死攻击感染的有效保护来体现。由于先前接触载体并未导致免疫抑制，我们得出结论，沙门氏菌鞭毛蛋白是合成肽基疫苗的合适载体。
