Blankenvoorde M F, Henskens Y M, van't Hof W, Veerman E C, Nieuw Amerongen A V
Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), The Netherlands.
Biol Chem. 1996 Dec;377(12):847-50.
The mutual effects of P. gingivalis and several cystatin species has been investigated. After incubation with P. gingivalis culture supernatant, cystatin S, cystatin C and chicken cystatin were truncated from a 14 kDa protein into a polypeptide of approximately 13 kDa. Amino acid sequence analysis of the truncated cystatin S polypeptide revealed that cystatin S was cleaved after Arg-8. All three types of truncated cystatins fully retained their inhibitory activity toward papain. Cystatin S and chicken cystatin partially inhibited proteolytic activity in the culture supernatant of P. gingivalis. Furthermore, cystatin S and chicken cystatin inhibited the growth of P. gingivalis in culture to 50% at approximately 1 microM.
