Fujikura T, Otsuka H
Department of Otorhinolaryngology-Head and Neck Surgery, Nippon Medical School, Dai 2 Hospital, Kawasaki, Kanagawa, Japan.
Arch Otolaryngol Head Neck Surg. 1998 Dec;124(12):1331-5. doi: 10.1001/archotol.124.12.1331.
To demonstrate the existence and localization of monocyte chemotactic and activating factor or monocyte chemoattractant protein-1 (MCAF/MCP-1) in human nasal mucosa and to verify its activity as a histamine-releasing factor.
Detection of MCAF/MCP-1 in culture supernatants of nasal mucosa using Western blot analysis and assay of histamine release from basophils induced by these culture supernatants. Detection of MCAF/MCP-1 expression in nasal mucosa of patients with perennial allergic rhinitis using immunohistochemistry.
Twenty-one patients with house dust mite allergy, 7 nonallergic patients, and 5 patients with chronic inflammatory sinusitis participated in the study. All the allergic patients had positive test results for mite nasal allergy, detected by a clinical history, a nasal provocation test, and determination of specific mite IgE antibodies by a radioallergosorbent test.
In Western blot analysis of supernatants of explant culture of human nasal mucosa, the band corresponding to approximately 13 to 15 kd was observed. This band was considered to be MCAF/MCP-1. These supernatants induced histamine release from basophils (approximately 3%-5% in net histamine release), and anti-MCAF/MCP-1 antibody inhibited this histamine-releasing activity. Immunoreactivity of MCAF/MCP-1 was observed in the nasal submucosa but not in the epithelium. Immunoreactive cells of MCAF/MCP-1 were also stained with the antibody, which recognizes monocytes and macrophages.
These results suggest that MCAF/MCP-1, which is produced constantly by monocytes and macrophages and is stored in human nasal mucosa, possibly participates in the protracted histamine release from basophils and in the pathogenesis of perennial allergic rhinitis.
证实单核细胞趋化激活因子或单核细胞趋化蛋白-1（MCAF/MCP-1）在人鼻黏膜中的存在及定位，并验证其作为组胺释放因子的活性。
采用蛋白质印迹分析法检测鼻黏膜培养上清液中的MCAF/MCP-1，并检测这些培养上清液诱导嗜碱性粒细胞释放组胺的情况。采用免疫组织化学法检测常年性变应性鼻炎患者鼻黏膜中MCAF/MCP-1的表达。
21例屋尘螨过敏患者、7例非过敏患者和5例慢性炎症性鼻窦炎患者参与了本研究。所有过敏患者经临床病史、鼻激发试验及放射性变应原吸附试验检测特异性螨IgE抗体，螨鼻过敏试验结果均为阳性。
在人鼻黏膜外植体培养上清液的蛋白质印迹分析中，观察到对应于约13至15 kd的条带。该条带被认为是MCAF/MCP-1。这些上清液可诱导嗜碱性粒细胞释放组胺（净组胺释放约3%-5%），抗MCAF/MCP-1抗体可抑制这种组胺释放活性。在鼻黏膜下层观察到MCAF/MCP-1的免疫反应性，而上皮中未观察到。MCAF/MCP-1的免疫反应性细胞也被识别单核细胞和巨噬细胞的抗体染色。
这些结果表明，由单核细胞和巨噬细胞持续产生并储存于人鼻黏膜中的MCAF/MCP-1可能参与嗜碱性粒细胞的持续性组胺释放及常年性变应性鼻炎的发病机制。
