Kuna P, Lazarovich M, Kaplan A P
Department of Medicine, State University of New York-Stony Brook 11794-8160, USA.
J Allergy Clin Immunol. 1996 Jan;97(1 Pt 1):104-12. doi: 10.1016/s0091-6749(96)70288-9.
Monocyte chemotactic and activating factor/monocyte chemoattractant protein-1 (MCAF/MCP-1), RANTES, and macrophage inflammatory protein (MIP)-1 alpha are chemokines known to activate basophils (MCAF/RANTES) and eosinophils (RANTES/MIP-1 alpha). IL-8 inhibits MCAF-induced histamine release from basophils. We questioned whether a relationship exists between the levels of these chemokines and various inflammatory mediators released from mast cells, eosinophils, and basophils as assessed in nasal secretions obtained from patients during the allergy season and out of season. Samples were assessed for MCAF/MCP-1, RANTES, MIP-1 alpha, IL-8, histamine, tryptase and eosinophil cationic protein (ECP) in three subject groups: subjects with allergic rhinitis (n = 18), atopic subjects without rhinitis (n = 9), and healthy individuals (n = 6). Statistically significant differences were apparent only in the subjects with symptoms as follows. MCAF/MCP-1 increased during the season from 336 +/- 47 pg/ml to 829 +/- 137 pg/ml (p < 0.001), whereas IL-8 decreased from a baseline of 1932 +/- 335 pg/ml to 1070 +/- 202 pg/ml (p < 0.028). The ratio of IL-8 to MCAF/MCP-1 decreased during the symptomatic season from the baseline of 6.66 +/- 1.06 seen during winter to 1.3 +/- 0.22 during ragweed season (p < 0.001). Histamine increased from 6.3 +/- 1.5 to 89 +/- 15.5 ng/ml (p < 0.001), ECP increased from 20.6 +/- 6.4 to 237.1 +/- 50.2 ng/ml (p < 0.001), and tryptase increased from 2.34 +/- 0.6 to 9.7 +/- 2.3 U/ml (p < 0.001). Most samples did not have detectable quantities of MIP-1 alpha or RANTES. We also found a correlation between the level of MCAF/MCP-1 and IL-8 and the level of histamine or IL-8 and ECP. Our results suggest that the chemokines MCAF/MCP-1 and IL-8 may participate in the pathogenesis of allergic rhinitis, contributing to the attraction of the proinflammatory cells and mediator release, which might be very important during the late phase of the allergic reaction. Furthermore, the ratio of certain chemokines, such as MCAF/MCP-1 and IL-8 may reflect the magnitude of the reaction, as does the presence of histamine and ECP.
单核细胞趋化激活因子/单核细胞趋化蛋白-1（MCAF/MCP-1）、调节激活正常T细胞表达和分泌的因子（RANTES）以及巨噬细胞炎性蛋白（MIP）-1α是已知可激活嗜碱性粒细胞（MCAF/RANTES）和嗜酸性粒细胞（RANTES/MIP-1α）的趋化因子。白细胞介素-8（IL-8）可抑制MCAF诱导的嗜碱性粒细胞组胺释放。我们想知道在过敏季节和非过敏季节从患者获取的鼻分泌物中评估的这些趋化因子水平与肥大细胞、嗜酸性粒细胞和嗜碱性粒细胞释放的各种炎性介质水平之间是否存在关联。对三个受试者组的样本进行了MCAF/MCP-1、RANTES、MIP-1α、IL-8、组胺、类胰蛋白酶和嗜酸性粒细胞阳离子蛋白（ECP）的评估：变应性鼻炎患者（n = 18）、无鼻炎的特应性受试者（n = 9）和健康个体（n = 6）。仅在有症状的受试者中出现了具有统计学意义的差异，具体如下。MCAF/MCP-1在季节期间从336±47 pg/ml增加至829±137 pg/ml（p < 0.001），而IL-8从基线水平1932±335 pg/ml降至1070±202 pg/ml（p < 0.028）。在有症状的季节，IL-8与MCAF/MCP-1的比值从冬季时的基线水平6.66±1.06降至豚草季节时的1.3±0.22（p < 0.001）。组胺从6.3±1.5增加至89±15.5 ng/ml（p < 0.001），ECP从20.6±6.4增加至237.1±50.2 ng/ml（p < 0.001），类胰蛋白酶从2.34±0.6增加至9.7±2.3 U/ml（p < 0.001）。大多数样本未检测到可检测量的MIP-1α或RANTES。我们还发现MCAF/MCP-1和IL-8的水平与组胺水平或IL-8和ECP的水平之间存在相关性。我们的结果表明，趋化因子MCAF/MCP-1和IL-8可能参与变应性鼻炎的发病机制，促进促炎细胞的吸引和介质释放，这在过敏反应的晚期可能非常重要。此外，某些趋化因子的比值，如MCAF/MCP-1和IL-8，可能反映反应的程度，组胺和ECP的存在情况也是如此。
