Matsunami K, Kokubo H, Ohno K, Suzuki Y
Graduate University for Advanced Studies, Okazaki, Japan.
Dev Growth Differ. 1998 Dec;40(6):591-7. doi: 10.1046/j.1440-169x.1998.t01-4-00003.x.
Embryonic and larval expression patterns of the sericin-1 gene and its presumed transcription factor, SGF-3/POU-M1, in the silk gland were analyzed by in situ hybridization and immunohistochemistry. The sericin-1 transcripts were first detected at embryonic stage 26 in an increasing gradient pattern in the middle and posterior part of the middle silk gland (MSG), while at the same stage the SGF-3/POU-M1 was already present in the entire anterior silk gland (ASG) and in the MSG but with a decreasing gradient pattern. The latter expression pattern was consistently maintained through all larval stages, while the sericin-1 expression was detected during the feeding stages but disappeared at the molting stages. These observations suggest that, although the SGF-3/POU-M1 was proposed to be a positive transcription factor for the sericin-1 gene, the protein might function in a negative manner on sericin-1 gene transcription. Alternatively, it is also possible that the sericin-1 gene might require another unidentified factor or mediator for in vivo transcription.
通过原位杂交和免疫组织化学分析了丝胶蛋白-1基因及其假定的转录因子SGF-3/POU-M1在丝腺中的胚胎和幼虫表达模式。丝胶蛋白-1转录本在胚胎发育第26阶段首次在中部丝腺（MSG）的中部和后部以递增梯度模式被检测到，而在同一阶段，SGF-3/POU-M1已经存在于整个前部丝腺（ASG）和MSG中，但呈递减梯度模式。后一种表达模式在所有幼虫阶段持续保持，而丝胶蛋白-1表达在取食阶段被检测到，但在蜕皮阶段消失。这些观察结果表明，尽管SGF-3/POU-M1被认为是丝胶蛋白-1基因的正转录因子，但该蛋白可能以负向方式作用于丝胶蛋白-1基因转录。或者，也有可能丝胶蛋白-1基因在体内转录可能需要另一种未鉴定的因子或介质。
