一种用于检测小麦胚芽体外蛋白质合成系统中白蛋白信使核糖核酸的分析方法。
An assay for albumin messenger RNA in an vitro protein synthesizing system from wheat germ.
作者信息
Peterson J A
出版信息
Nucleic Acids Res. 1976 Jun;3(6):1427-36. doi: 10.1093/nar/3.6.1427.
Abstract
The synthesis of serum albumin in an in vitro protein-synthesizing system from wheat germ stimulated with rat liver polysomal RNA is demonstrated by immunoprecipitation. The newly synthesized albumin has the same electrophoretic mobility as rat serum albumin. There is a linear increase in precursor incorporation into total protein and albumin with increasing RNA concentration. Potassium and magnesium optima for albumin synthesis are different from those for total protein synthesis.
摘要
通过免疫沉淀法证明，在用大鼠肝脏多聚核糖体RNA刺激的小麦胚芽体外蛋白质合成系统中，血清白蛋白能够合成。新合成的白蛋白与大鼠血清白蛋白具有相同的电泳迁移率。随着RNA浓度的增加，前体掺入总蛋白和白蛋白的量呈线性增加。白蛋白合成的钾离子和镁离子最佳浓度与总蛋白合成的不同。
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本文引用的文献
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Ann N Y Acad Sci. 1964 Dec 28;121:404-27. doi: 10.1111/j.1749-6632.1964.tb14213.x.
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Characterization of a highly efficient protein synthesizing system derived from commercial wheat germ.源自市售小麦胚芽的高效蛋白质合成系统的特性分析
Nucleic Acids Res. 1974 Nov;1(11):1385-97. doi: 10.1093/nar/1.11.1385.
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Serum albumin.血清白蛋白
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