Ingole K V, Jalgaonkar S V, Fule R P
Department of Microbiology, V.N. Govt. Medical College, Yavatmal.
Indian J Pathol Microbiol. 1998 Oct;41(4):419-22.
V. cholerae 01 E1 Tor isolated during Cholera epidemic of 1994 and V cholerae 0139 serotype isolated during 1993 epidemic were subjected to the study of proteases and other enzymes. Out of 26 strains of V. cholerae 01 studied, gelatinase and caseinase activity was seen in 100 and 69.23 percent strains respectively. All strains showed catalase and oxidase activity. Of the other enzymes studied 19.23, 65.38 and 57.69 percent strains were positive for DNAse, lipase and phosphatase respectively. None of the strains showed lecithinase activity. Similar findings were observed in 22 strains of V. cholerae 0139 except all strains were positive for phosphatase activity. Role of enzymes in virulence is suggested.
对1994年霍乱流行期间分离出的霍乱弧菌O1 El Tor菌株以及1993年流行期间分离出的霍乱弧菌O139血清型菌株进行了蛋白酶和其他酶的研究。在所研究的26株霍乱弧菌O1菌株中，分别有100%和69.23%的菌株具有明胶酶和酪蛋白酶活性。所有菌株均表现出过氧化氢酶和氧化酶活性。在所研究的其他酶中，分别有19.23%、65.38%和57.69%的菌株对脱氧核糖核酸酶、脂肪酶和磷酸酶呈阳性反应。所有菌株均未表现出卵磷脂酶活性。在22株霍乱弧菌O139中观察到了类似的结果，只是所有菌株的磷酸酶活性均呈阳性。提示了酶在毒力中的作用。
