Qian H, Sheng M
Department of Foods and Nutrition, The University of Georgia, Athens 30602, USA.
J Chromatogr A. 1998 Nov 6;825(2):127-33. doi: 10.1016/s0021-9673(98)00733-x.
Traditionally, alkaline saponification for the extraction of vitamins and step-wise HPLC analyses have been widely used for analysis of fat-soluble vitamins in animal feeds. The objective of present study was to develop an analytical method using one-step extraction and simultaneous determination of the vitamins A, D and E and pro-vitamin D2 in animals feeds b HPLC. Various analytical conditions were tested which included sample particle size extraction solvents, the ratio of solvent to sample, extraction approach, extraction time, N2 protection, prepurification with Sep-Pak cartridge and detection wavelength. The accuracy of the developed method has been proven by comparison with the AOAC method. The reproducibility of the developed method has been substantiated by repeated recovery experiments. The detection limit for the four vitamins was 10 ng/g of feed sample. One of the important properties of the present method is rapidity and ease of use.
传统上，用于提取维生素的碱性皂化法和逐步高效液相色谱分析已被广泛用于分析动物饲料中的脂溶性维生素。本研究的目的是开发一种采用一步萃取法，通过高效液相色谱同时测定动物饲料中维生素A、D、E和维生素原D2的分析方法。测试了各种分析条件，包括样品粒度、萃取溶剂、溶剂与样品的比例、萃取方法、萃取时间、氮气保护、用Sep-Pak柱进行预纯化以及检测波长。通过与美国官方分析化学师协会（AOAC）的方法进行比较，证明了所开发方法的准确性。通过重复回收率实验证实了所开发方法的重现性。这四种维生素的检测限为每克饲料样品10纳克。本方法的一个重要特性是快速且易于使用。
