Ake M, Fabre H, Malan A K, Mandrou B
Laboratoire de Chimie Analytique, Faculté de Pharmacie, Montpellier, France.
J Chromatogr A. 1998 Nov 27;826(2):183-9. doi: 10.1016/s0021-9673(98)00747-x.
A high-performance liquid chromatography method was developed for the simultaneous routine determination of vitamins A and E in powdered milk and flour made from local plants and purchased from open markets in the Ivory Coast. The method involves saponification followed by extraction with a mixture of organic solvents. The vitamins were resolved by reversed-phase HPLC and detection at a single wavelength. The main tests of method validation were applied to the procedure. The results show the reliability of the analytical method for the intended application.
开发了一种高效液相色谱法，用于同时常规测定从科特迪瓦公开市场购买的当地植物制成的奶粉和面粉中的维生素A和维生素E。该方法包括皂化，然后用有机溶剂混合物萃取。通过反相高效液相色谱法分离维生素，并在单一波长下进行检测。对该方法进行了主要的方法验证测试。结果表明该分析方法对于预期应用具有可靠性。
