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烟曲霉利用麦芽糖的合成类似物α-甲基-D-糖苷作为底物生产淀粉酶。

Production of amylase by Aspergillus fumigatus utilizing alpha-methyl-D-glycoside, a synthetic analogue of maltose, as substrate.

作者信息

Goto C E, Barbosa E P, Kistner L C, Moreira F G, Lenartovicz V, Peralta R M

机构信息

Departamento de Bioquímica, Universidade Estadual de Maringá, Brazil.

出版信息

FEMS Microbiol Lett. 1998 Oct 15;167(2):139-43. doi: 10.1111/j.1574-6968.1998.tb13219.x.

Abstract

A strain of Aspergillus fumigatus isolated from soil was able to produce biomass and high amylase activities in media containing alpha-methyl-D-glucoside (alphaMG), a synthetic analogue of maltose, as the only carbon source. alphaMG was a more effective inducer than starch and maltose at the same concentration: alphaMG cultures produced about 3 times more alpha-amylase and glucoamylase activity than starch cultures. Maximum production of alpha-amylase (60 U/mg) and glucoamylase (130 U/mg) was obtained in 8-10 days alphaMG cultures.

摘要

从土壤中分离出的一株烟曲霉能够在以麦芽糖的合成类似物α-甲基-D-葡萄糖苷(αMG)作为唯一碳源的培养基中产生生物量并具有高淀粉酶活性。在相同浓度下,αMG比淀粉和麦芽糖是更有效的诱导剂:αMG培养物产生的α-淀粉酶和葡糖淀粉酶活性比淀粉培养物高约3倍。在αMG培养8 - 10天可获得α-淀粉酶(60 U/mg)和葡糖淀粉酶(130 U/mg)的最大产量。

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