Ward G R, Huang Y S, Bobik E, Xing H C, Mutsaers L, Auestad N, Montalto M, Wainwright P
Department of Health Studies and Gerontology, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1.
J Nutr. 1998 Dec;128(12):2473-87. doi: 10.1093/jn/128.12.2473.
We studied the effects of dietary long-chain polyunsaturated fatty acids (PUFA) on the fatty acid composition of the brain and red blood cells in gastrostomized rat pups reared artificially from postnatal Days 5-18. These pups were fed rat milk substitutes in which the fat comprised 10% linoleic acid and 1% alpha-linolenic acid and, using a 3 x 3 factorial design, one of three levels of both arachidonic acid (AA) and docosahexaenoic acid (DHA) supplied as single cell microbial oils (0.0, 0.4 and 2.4% fatty acids). A tenth group was reared by nursing dams. The fatty acid composition of the phosphatidylethanolamine (PE) and phosphatidylserine/phosphatidylinositol (PS/PI) phospholipids in the brain and red blood cells on Day 18 reflected the dietary composition in that pups receiving long-chain supplementation of each had higher levels of the supplemented PUFA, but lower levels of the other, relative to unsupplemented groups. In contrast to these results, there were few changes in the brain in phosphatidylcholine (PC) phospholipids whereas, in the red blood cells, changes in PC were similar to those in PE and PS/PI. Regression analyses showed that DHA levels in the brain correlated more closely with those of the red blood cells than did AA levels. The results of this study indicate that, although supplementation of formula with AA or DHA during the period of rapid brain development in rats increases deposition of the long-chain PUFA in the developing tissues, each also affects the levels of the other.
我们研究了膳食长链多不饱和脂肪酸（PUFA）对出生后第5至18天人工饲养的胃造口大鼠幼崽大脑和红细胞脂肪酸组成的影响。这些幼崽喂食的大鼠代乳品中脂肪包含10%的亚油酸和1%的α-亚麻酸，并采用3×3析因设计，以单细胞微生物油形式提供三种水平的花生四烯酸（AA）和二十二碳六烯酸（DHA）之一（0.0、0.4和2.4%脂肪酸）。第十组由哺乳母鼠饲养。第18天大脑和红细胞中磷脂酰乙醇胺（PE）以及磷脂酰丝氨酸/磷脂酰肌醇（PS/PI）磷脂的脂肪酸组成反映了饮食组成，即相对于未补充组，接受每种长链脂肪酸补充的幼崽中，补充的PUFA水平较高，但另一种的水平较低。与这些结果相反，大脑中磷脂酰胆碱（PC）磷脂几乎没有变化，而在红细胞中，PC的变化与PE和PS/PI相似。回归分析表明，大脑中DHA水平与红细胞中DHA水平的相关性比AA水平更密切。本研究结果表明，尽管在大鼠大脑快速发育期间，在配方奶中补充AA或DHA会增加发育组织中长链PUFA的沉积，但每种补充剂也会影响另一种的水平。
