Hwang G S, Kim J Y, Chang T S, Jeon S D, So D S, Moon C K
College of Oriental Medicine, Kyung Won University, Seongnam, Korea.
Arch Pharm Res. 1998 Dec;21(6):774-8. doi: 10.1007/BF02976775.
Brazilin (7,11b-dihydrobenz[b]indeno[1,2-d]pyran-3,6a,9,10 (6 H)-tetrol) inhibited thrombin-,collagen- and ADP-induced aggregation of washed rat platelets. Thrombin- and collagen-induced ATP release were also inhibited by brazilin in a concentration-dependent manner. Brazilin inhibited the formation of platelet thromboxane A2 caused by thrombin, whereas it had no effect on the prostaglandin D2 formation. Brazilin inhibited [3H]-arachidonic acid liberation from membrane phospholipids of thrombin-stimulated platelets. Brazilin inhibited the rise of intracellular free calcium caused by thrombin. These results indicate that the inhibition of phospholipase (PLA2) activity and [Ca2+]i elevation might be at least a part of antiplatelet mechanism of brazilin.
巴西苏木精（7,11b - 二氢苯并[b]茚并[1,2 - d]吡喃 - 3,6a,9,10（6H）- 四醇）抑制凝血酶、胶原和ADP诱导的洗涤大鼠血小板聚集。巴西苏木精还以浓度依赖性方式抑制凝血酶和胶原诱导的ATP释放。巴西苏木精抑制凝血酶引起的血小板血栓素A2的形成，而对前列腺素D2的形成没有影响。巴西苏木精抑制凝血酶刺激的血小板膜磷脂中[3H] - 花生四烯酸的释放。巴西苏木精抑制凝血酶引起的细胞内游离钙升高。这些结果表明，抑制磷脂酶（PLA2）活性和细胞内钙离子浓度升高可能至少是巴西苏木精抗血小板机制的一部分。
