Constans J, Seigneur M, Blann A D, Renard M, Resplandy F, Amiral J, Guérin V, Boisseau M R, Conri C
Service de Médecine Interne et Pathologie Vasculaire, Hôpital St. André, Bordeaux, France.
Thromb Haemost. 1998 Dec;80(6):1015-7.
Patients infected with HIV are at increased risk of atherosclerosis, and have evidence of endothelium dysfunction. The hypothesis was tested that HIV-related endothelium dysfunction is related to loss of antioxidants. This was done by the supplementation of the antioxidants selenium and beta-carotene. We supplemented the diet of 10 HIV-seropositive subjects with 100 microg selenium daily, 11 subjects with 30 mg beta-carotene twice daily while 15 subjects were not supplemented. Plasma was obtained at outset and after a year, and tested by ELISA for endothelial cell, platelet and inflammatory markers. The non-supplemented patients experienced increases in von Willebrand factor and soluble thrombomodulin (both p <0.01). There were no changes in any of the indices in the patients taking selenium or beta-carotene. Increased von Willebrand factor and soluble thrombomodulin in the non-supplemented patients imply increased damage to the endothelium over the year of the study. Therefore we interpret the lack of increase in the patients taking antioxidants as evidence of the protection of the endothelium by these agents.
感染艾滋病毒的患者患动脉粥样硬化的风险增加，且有内皮功能障碍的证据。我们检验了一个假设，即与艾滋病毒相关的内皮功能障碍与抗氧化剂的缺失有关。我们通过补充抗氧化剂硒和β-胡萝卜素来进行验证。我们给10名艾滋病毒血清呈阳性的受试者每天补充100微克硒，给11名受试者每天两次补充30毫克β-胡萝卜素，而15名受试者不进行补充。在研究开始时和一年后采集血浆，并用酶联免疫吸附测定法检测内皮细胞、血小板和炎症标志物。未补充的患者血管性血友病因子和可溶性血栓调节蛋白增加（两者p<0.01）。服用硒或β-胡萝卜素的患者各项指标均无变化。在未补充的患者中，血管性血友病因子和可溶性血栓调节蛋白增加意味着在研究的一年中内皮损伤增加。因此，我们将服用抗氧化剂的患者未出现增加解释为这些药物对内皮有保护作用的证据。
