羟苯磺酸钙对自发性糖尿病大鼠主动脉反应性的体外作用

In vitro effects of calcium dobesilate on the responsiveness of spontaneously diabetic rat aorta.

作者信息

Sanz M, Ruiz E, Ganado P, Tejerina T

机构信息

Department of Pharmacology, School of Medicine, Complutense University, Madrid, Spain.

出版信息

Jpn J Pharmacol. 1998 Nov;78(3):391-4. doi: 10.1254/jjp.78.391.

DOI:10.1254/jjp.78.391

PMID:9869275

Abstract

We tested the effect of calcium dobesilate (DOBE) in aorta from spontaneously diabetic (BB/wor) rats. The contraction induced by 10(-6) M noradrenaline (NA) in BB/wor rats was smaller than that induced in control rats (1.21+/-0.11 vs 0.82+/-0.02 g, P<0.01, n=8, respectively) in arteries with intact endothelium. Incubation with DOBE (10(-4) M) impaired the contractions induced by NA in BB/wor rats (1.21+/-0.11 vs 0.67+/-0.01 g, P<0.01, n=8). The effect of DOBE was reversed by 10(-6) M propranolol (0.67+/-0.01 vs 1.20+/-0.60g, P<0.001, n=8, with 10(-4) M DOBE and 10(-4)M DOBE plus 10(-6) M propranolol, respectively). DOBE increased the endothelium-dependent relaxation in arteries from diabetic rats. These findings suggest that DOBE might improve vascular reactivity in BB/wor rats.

摘要

我们检测了羟苯磺酸钙（DOBE）对自发性糖尿病（BB/wor）大鼠主动脉的作用。在具有完整内皮的动脉中，10⁻⁶M去甲肾上腺素（NA）诱导的BB/wor大鼠的收缩小于对照大鼠（分别为1.21±0.11g与0.82±0.02g，P<0.01，n = 8）。用DOBE（10⁻⁴M）孵育会削弱NA诱导的BB/wor大鼠的收缩（1.21±0.11g与0.67±0.01g，P<0.01，n = 8）。10⁻⁶M普萘洛尔可逆转DOBE的作用（分别为0.67±0.01g与1.20±0.60g，P<0.001，n = 8，分别使用10⁻⁴M DOBE和10⁻⁴M DOBE加10⁻⁶M普萘洛尔）。DOBE增加了糖尿病大鼠动脉中内皮依赖性舒张。这些发现表明DOBE可能改善BB/wor大鼠的血管反应性。