Ishibashi T, Murata T, Hangai M, Nagai R, Horiuchi S, Lopez P F, Hinton D R, Ryan S J
Department of Ophthalmology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Arch Ophthalmol. 1998 Dec;116(12):1629-32. doi: 10.1001/archopht.116.12.1629.
To investigate the localization of N epsilon-(carboxymethyl)lysine (CML), a component and major immunologic epitope of advanced glycation end products, in aged eyes and choroidal neovascular membranes (CNVMs) surgically excised from eyes with age-related macular degeneration.
Immunohistochemistry for CML was performed using 8 snap-frozen, surgically excised CNVMs. Twelve eyes from patients aged 69 to 82 years and 2 donor eyes, 1 each from a 23-week-old fetus and 21-year-old patient, without age-related macular degeneration or diabetic retinopathy were also examined. To determine if retinal pigment epithelial cells in CNVMs accumulate advanced glycation end products, cytokeratin and CML were stained in paired serial sections.
Soft, macular drusen and/or basal laminar and basal linear deposits were observed in 8 of 12 aged eyes. Each case showed CML accumulation, while overlying retinal pigment epithelial cells showed no accumulation in all 12 eyes. In CNVMs, however, retinal pigment epithelial cells showed CML accumulation in their cytoplasm.
The additional accumulation of advanced glycation end products in soft, macular drusen and/or retinal pigment epithelial cells may play a role in the pathogenesis of CNVM formation in age-related macular degeneration.
Recently, advanced glycation end products have been found to play a role both in aging changes and neovascularization. Localization of advanced glycation end products in the above-mentioned tissue may lead to a better understanding of the pathogenesis of age-related macular degeneration.
研究晚期糖基化终末产物的成分及主要免疫表位Nε-（羧甲基）赖氨酸（CML）在老年眼中以及从年龄相关性黄斑变性患者眼中手术切除的脉络膜新生血管膜（CNVM）中的定位。
使用8个手术切除的CNVM冰冻切片进行CML免疫组织化学检测。还检查了12例年龄在69至82岁患者的眼睛以及2只供体眼，供体眼分别来自一名23周龄的胎儿和一名21岁的患者，均无年龄相关性黄斑变性或糖尿病性视网膜病变。为了确定CNVM中的视网膜色素上皮细胞是否积累晚期糖基化终末产物，在配对的连续切片中对细胞角蛋白和CML进行染色。
12只老年眼中有8只观察到软性黄斑玻璃膜疣和/或基底膜及基底线性沉积物。每例均显示CML积累，而在所有12只眼中，上方的视网膜色素上皮细胞均未显示积累。然而，在CNVM中，视网膜色素上皮细胞的细胞质中显示有CML积累。
晚期糖基化终末产物在软性黄斑玻璃膜疣和/或视网膜色素上皮细胞中的额外积累可能在年龄相关性黄斑变性中CNVM形成的发病机制中起作用。
最近发现晚期糖基化终末产物在衰老变化和新生血管形成中均起作用。晚期糖基化终末产物在上述组织中的定位可能有助于更好地理解年龄相关性黄斑变性的发病机制。
