Kwak J Y, Gilman-Sachs A, Moretti M, Beaman K D, Beer A E
FUHS/The Chicago Medical School, Department of Microbiology and Immunology, North Chicago, Illinois 60064, USA.
Am J Reprod Immunol. 1998 Nov;40(5):352-8. doi: 10.1111/j.1600-0897.1998.tb00065.x.
Natural killer (NK)-cell cytotoxicity in women undergoing lymphocyte immunization prior to and following treatment was investigated.
A cohort of 33 women with a history of two or more recurrent spontaneous abortions was prospectively studied. NK-cell cytotoxicity was determined at effector-to-target ratios of 50:1 and 25:1. Peripheral blood CD56+ NK-cell, CD19+ B-cell, CD19+/5+ B-1-cell, and CD3+ pan T-cell levels were studied by flow cytometry before and after lymphocyte immunization treatment. Maternal antipaternal T- and B-cell antibody levels were measured before and after lymphocyte immunization by flow cytometric analysis. Paternal lymphocyte immunizations were given two times with a 4-week interval. Post-lymphocyte immunization testing was done 4 weeks after the second lymphocyte immunization. The controls were 8 normal healthy women. NK assays were done twice with an interval of 8 weeks.
NK-cell activity at effector-to-target ratios of 50:1 (P = 0.005) and 25:1 (P = 0.001) were significantly suppressed after lymphocyte immunization. CD3+ pan T-cell levels after lymphocyte immunization were significantly increased compared with levels before lymphocyte immunization (P = 0.008). CD56+ NK-cell levels were significantly suppressed after lymphocyte immunization (P = 0.016). There was no correlation between changes in NK cytotoxicity and differences in antipaternal lymphocyte antibody levels before or after lymphocyte immunization.
Lymphocyte immunization suppresses NK-cell cytotoxicity and CD56+ NK-cell levels and increases the peripheral blood CD3+ T-cell population in women with recurrent spontaneous abortions.
对接受淋巴细胞免疫治疗前后的女性自然杀伤（NK）细胞细胞毒性进行了研究。
对33例有两次或更多次复发性自然流产病史的女性进行前瞻性研究。在效应细胞与靶细胞比例为50:1和25:1时测定NK细胞细胞毒性。通过流式细胞术研究淋巴细胞免疫治疗前后外周血CD56⁺NK细胞、CD19⁺B细胞、CD19⁺/5⁺B-1细胞和CD3⁺全T细胞水平。通过流式细胞术分析在淋巴细胞免疫前后测量母体抗父本T细胞和B细胞抗体水平。父本淋巴细胞免疫每隔4周进行两次。在第二次淋巴细胞免疫后4周进行淋巴细胞免疫后检测。对照组为8名正常健康女性。NK检测每隔8周进行两次。
淋巴细胞免疫后，效应细胞与靶细胞比例为50:1（P = 0.005）和25:1（P = 0.001）时的NK细胞活性受到显著抑制。淋巴细胞免疫后的CD3⁺全T细胞水平与淋巴细胞免疫前相比显著升高（P = 0.008）。淋巴细胞免疫后CD56⁺NK细胞水平受到显著抑制（P = 0.016）。NK细胞毒性的变化与淋巴细胞免疫前后抗父本淋巴细胞抗体水平的差异之间没有相关性。
淋巴细胞免疫可抑制复发性自然流产女性的NK细胞细胞毒性和CD56⁺NK细胞水平，并增加外周血CD3⁺T细胞数量。
