Taylor R B, Toasaksiri S, Reid R G
School of Pharmacy, The Robert Gordon University, Schoolhill, Aberdeen, UK.
Electrophoresis. 1998 Nov;19(16-17):2791-7. doi: 10.1002/elps.1150191606.
A literature survey on published reports of the determination of drugs in biological fluids shows that all methods of sample pretreatment have been used and that the limits of detection achieved vary widely, ranging from low ngcm(-3) to microgcm(-3). The most widely used injection method was hydrodynamic and, in the majority of cases, whenever low detection limits were achieved, this was a result of preconcentration during the sample pretreatment. Only a small proportion of the reported methods employed electrokinetic injection and utilised the field amplified sample injection (FASI) techniques. An experimental investigation of the alternative hydrodynamic and electrokinetic injection methods for a small set of antimalarial drugs is reported. It was found that electrokinetic injection with FASI from an acetonitrile-water matrix produced dramatic improvements in detection limits. This improvement could not, however, be achieved when the drugs were in plasma using protein precipitation, liquid-liquid extraction or solid phase extraction pretreatment methods. This highlights the importance of sample pretreatment in utilising the potential sensitivity of capillary electrophoresis with electrokinetic injection.
一项关于生物流体中药物测定的已发表报告的文献调查表明，所有样品预处理方法均已被使用，且所达到的检测限差异很大，范围从低纳克每立方厘米（ngcm⁻³）到微克每立方厘米（μgcm⁻³）。使用最广泛的进样方法是流体动力学进样，并且在大多数情况下，只要达到低检测限，这都是样品预处理过程中预富集的结果。所报道的方法中只有一小部分采用电动进样并利用了场放大样品进样（FASI）技术。本文报道了对一小类抗疟药物采用流体动力学进样和电动进样这两种替代方法的实验研究。结果发现，在乙腈 - 水基质中采用FASI进行电动进样可显著提高检测限。然而，当使用蛋白质沉淀、液 - 液萃取或固相萃取预处理方法处理血浆中的药物时，无法实现这种改善。这突出了样品预处理在利用电动进样毛细管电泳潜在灵敏度方面的重要性。
