Lloyd D K
Meakins-Christie Laboratories, McGill University, Montreal, Quebec, Canada.
J Chromatogr A. 1996 May 31;735(1-2):29-42. doi: 10.1016/0021-9673(95)01350-4.
A variety of strategies for the analysis of biological samples by capillary electrophoresis (CE) are described, with particular emphasis on the determination of drugs and metabolites. Analytical methods involving extensive sample pretreatment before CE analysis are considered, as well as strategies for directly injecting untreated biofluids. The application in CE of techniques common in liquid chromatography is first described, e.g. protein precipitation, liquid-liquid extraction and solid-phase extraction. On-capillary methods of sample concentration are considered. Approaches to performing CE assays of urine and plasma, without prior sample treatment, are described. The use of both capillary zone electrophoresis and micellar electrokinetic chromatography for direct-injection assays is compared for both urine and plasma analyses, and capillary washing strategies are discussed. Finally, direct-injection microanalyses are mentioned.
描述了通过毛细管电泳（CE）分析生物样品的多种策略，特别强调了药物和代谢物的测定。考虑了在CE分析之前进行大量样品预处理的分析方法，以及直接注入未处理生物流体的策略。首先描述了液相色谱中常用技术在CE中的应用，例如蛋白质沉淀、液液萃取和固相萃取。考虑了毛细管上的样品浓缩方法。描述了在不进行样品预处理的情况下对尿液和血浆进行CE分析的方法。比较了毛细管区带电泳和胶束电动色谱在尿液和血浆分析中直接进样分析的应用，并讨论了毛细管清洗策略。最后提到了直接进样微量分析。
