Bedford M, Arman E, Orr-Urtreger A, Lonai P
Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, Israel.
DNA Cell Biol. 1995 Apr;14(4):295-304. doi: 10.1089/dna.1995.14.295.
This study set out to investigate the structure and localized expression of the mouse homeobox-containing gene Hoxd-3. In addition to identifying a transcript of the type known from other Antennapedia (Antp)-like mammalian homeobox cDNAs, an antisense transcript was also detected. The antisense form of Hoxd-3 overlaps with 603 bp of the sense transcript including the homeobox. Active antisense transcription has been confirmed by RNA blot analysis with single-stranded probes and by the direction of splicing of an intron in the antisense transcript. The localized expression of sense and antisense transcripts was compared by in situ hybridization. Hoxd-3 expression was observed from 8.5 days p.c., in the neural tube with a sharp border in the hind brain at the level of rhombomeres 4-5. In contrast, the earliest antisense expression was detected at 10.5 days p.c. in cDNA libraries. At 12.5 days p.c., sense and antisense transcripts colocalized in the liver. The possible role of antisense homeobox transcripts during liver and the hematopoietic development is discussed.
本研究旨在探究小鼠含同源框基因Hoxd - 3的结构及局部表达情况。除了鉴定出一种与其他类触角足（Antp）的哺乳动物同源框cDNA已知类型相同的转录本外，还检测到了一种反义转录本。Hoxd - 3的反义形式与包括同源框在内的有义转录本的603个碱基对重叠。通过使用单链探针的RNA印迹分析以及反义转录本中一个内含子的剪接方向，证实了存在活跃的反义转录。通过原位杂交比较了有义转录本和反义转录本的局部表达情况。在胚胎发育第8.5天，在神经管中观察到Hoxd - 3的表达，在后脑菱脑节4 - 5水平处有明显边界。相比之下，最早在胚胎发育第10.5天的cDNA文库中检测到反义表达。在胚胎发育第12.5天，有义转录本和反义转录本在肝脏中共定位。讨论了反义同源框转录本在肝脏和造血发育过程中的可能作用。
