Gálvez A, Valdivia E, Abriouel H, Camafeita E, Mendez E, Martínez-Bueno M, Maqueda M
Departamento de Microbiología, Facultad de Ciencias Experimentales, Universidad de Jaén, E-23071 Jaén, Spain.
Arch Microbiol. 1998 Dec;171(1):59-65. doi: 10.1007/s002030050678.
The bacteriocinogenic strain of Enterococcus faecalis EJ97 has been isolated from municipal waste water. It produces a cationic bacteriocin (enterocin EJ97) of low molecular mass (5,340 Da) that is very stable under mild heat conditions and is sensitive to proteolytic enzymes. The amino acid sequence of the first 18 N-terminal residues of enterocin EJ97 indicates that it is different from other known protein sequences. Enterocin EJ97 is active on several gram-positive bacteria including enterococci, several species of Bacillus, Listeria, and Staphylococcus aureus. The producer strain is immune to bacteriocin. Enterocin EJ97 has a concentration-dependent bactericidal and bacteriolytic effect on E. faecalis S-47.
粪肠球菌EJ97的产细菌素菌株是从城市废水中分离出来的。它产生一种低分子量（5340道尔顿）的阳离子细菌素（肠球菌素EJ97），该细菌素在温和的加热条件下非常稳定，并且对蛋白水解酶敏感。肠球菌素EJ97前18个N端残基的氨基酸序列表明它与其他已知蛋白质序列不同。肠球菌素EJ97对几种革兰氏阳性菌有活性，包括肠球菌、几种芽孢杆菌属、李斯特菌和金黄色葡萄球菌。产生菌对细菌素具有免疫性。肠球菌素EJ97对粪肠球菌S-47具有浓度依赖性的杀菌和溶菌作用。
