粪肠球菌RJ-11产生的一种新型细菌素的纯化与特性分析
Purification and characterization of a novel bacteriocin produced by Enterococcus faecalis strain RJ-11.
作者信息
Yamamoto Yukio, Togawa Yoshikazu, Shimosaka Makoto, Okazaki Mitsuo
机构信息
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano 386-8567, Japan.
出版信息
Appl Environ Microbiol. 2003 Oct;69(10):5746-53. doi: 10.1128/AEM.69.10.5546-5553.2003.
Abstract
Lactic acid bacteria exhibiting activity against the gram-positive bacterium Bacillus subtilis were isolated from rice bran. One of the isolates, identified as Enterococcus faecalis RJ-11, exhibited a wide spectrum of growth inhibition with various gram-positive bacteria. A bacteriocin purified from culture fluid, designated enterocin RJ-11, was heat stable and was not sensitive to acid and alkaline conditions, but it was sensitive to several proteolytic enzymes. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis revealed that enterocin RJ-11 had a molecular weight of 5,000 in its monomeric form. The amino acid sequence determined for purified enterocin RJ-11 exhibited high levels of similarity to the sequences of enterocins produced by Enterococcus faecium.
摘要
从米糠中分离出了对革兰氏阳性菌枯草芽孢杆菌具有活性的乳酸菌。其中一株分离菌被鉴定为粪肠球菌RJ - 11，它对多种革兰氏阳性菌表现出广泛的生长抑制作用。从培养液中纯化得到的一种细菌素，命名为肠球菌素RJ - 11，具有热稳定性，对酸性和碱性条件不敏感，但对几种蛋白水解酶敏感。十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳分析表明，肠球菌素RJ - 11的单体形式分子量为5000。纯化的肠球菌素RJ - 11的氨基酸序列与屎肠球菌产生的肠球菌素序列具有高度相似性。
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