Grellner W, Dimmeler S, Madea B
Institute of Forensic Medicine, University of Bonn, Germany.
Forensic Sci Int. 1998 Nov 9;97(2-3):109-16. doi: 10.1016/s0379-0738(98)00147-9.
Fibronectin plays an important role in tissue repair and wound healing. Previous literature reports indicated that fibronectin could be a marker of vitality for wounds with a survival time of more than a few minutes. In order to verify these findings were performed immunohistochemical investigations on the expression of fibronectin in incised wounds of porcine skin inflicted into various anatomical regions in the early postmortem interval (0-5 min after circulatory arrest). Tissue specimens were collected 12-14 h postmortem and investigated for fibronectin on paraffin sections (alkaline phosphatase and monoclonal anti-alkaline phosphatase procedure). A markedly positive expression of fibronectin (not only next to the wound margin and clearly stronger than in normal undamaged skin) could be demonstrated in eleven out of 36 samples. A moderately positive fibronectin reaction was detectable in seven further specimens. Muscular contraction bands were observed in nine out of 36 specimens. The various reaction patterns of fibronectin cannot be finally explained, but are probably due to a passive transudation of skin tissue by blood components from injured vessels. As a result of these investigations the specificity and validity of fibronectin as a parameter of vitality has to be questioned. Fibronectin immunohistochemistry again reveals that the validity of parameters as vital criteria decreases when their manifestation time is short; postmortem/supravital induction seems to be possible resembling vital reactions in these cases.
纤连蛋白在组织修复和伤口愈合中发挥着重要作用。以往文献报道表明，纤连蛋白可能是存活时间超过几分钟的伤口活力的标志物。为了验证这些发现，对猪皮肤在死后早期（循环停止后0 - 5分钟）不同解剖区域造成的切割伤口中纤连蛋白的表达进行了免疫组织化学研究。在死后12 - 14小时收集组织标本，并在石蜡切片上检测纤连蛋白（碱性磷酸酶和单克隆抗碱性磷酸酶方法）。在36个样本中的11个样本中可显示出纤连蛋白的明显阳性表达（不仅在伤口边缘附近，且明显强于正常未受损皮肤）。在另外7个标本中可检测到中等程度的纤连蛋白阳性反应。在36个标本中的9个标本中观察到肌肉收缩带。纤连蛋白的各种反应模式尚无法最终解释，但可能是由于受损血管中的血液成分对皮肤组织的被动渗出所致。这些研究结果表明，纤连蛋白作为活力参数的特异性和有效性值得质疑。纤连蛋白免疫组织化学再次表明，当参数的表现时间较短时，作为重要标准的参数的有效性会降低；在这些情况下，死后/生前诱导似乎是可能的，类似于生命反应。
