Fouquet J P, Kann M L, Combeau C, Melki R
Laboratoire de Biologie Cellulaire, Groupe Spermatogenèse et Maturation du Spermatozoïde, Université Paris V, UFR Biomédicale, France.
Mol Hum Reprod. 1998 Dec;4(12):1122-9. doi: 10.1093/molehr/4.12.1122.
The distribution of gamma-tubulin as a marker of microtubule organizing centres (MTOC) was studied during spermiogenesis in rodents and in rabbit, monkey and man. A polyclonal antibody directed against human gamma-tubulin was used both for indirect immunofluorescence (IIF) and post-embedding immunogold procedures. In all species, gamma-tubulin was detected in the proximal and distal centrioles of round spermatids. In elongating spermatids, gamma-tubulin was predominantly found in the pericentriolar material (PCM) of both centrioles and particularly around the adjunct of the proximal centriole. At this level, some labelling was also associated with manchette microtubules, but other parts of the manchette and the nuclear ring were never labelled. We propose a role for distal centriole gamma-tubulin in axoneme nucleation and centriolar adjunct gamma-tubulin in manchette nucleation. The disappearance of gamma-tubulin in mature spermatozoa indicates that sperm aster nucleation should be dependent on oocyte gamma-tubulin. Remnants of gamma-tubulin in some human spermatozoa suggest that paternal gamma-tubulin also could contribute to sperm aster formation.
在啮齿动物、兔、猴和人的精子发生过程中，研究了作为微管组织中心（MTOC）标志物的γ-微管蛋白的分布。一种针对人γ-微管蛋白的多克隆抗体用于间接免疫荧光（IIF）和包埋后免疫金程序。在所有物种中，在圆形精子细胞的近端和远端中心粒中都检测到了γ-微管蛋白。在伸长的精子细胞中，γ-微管蛋白主要存在于两个中心粒的中心粒周围物质（PCM）中，特别是在近端中心粒的附属物周围。在此水平上，一些标记也与袖套微管相关，但袖套的其他部分和核环从未被标记。我们提出远端中心粒γ-微管蛋白在轴丝成核中的作用以及中心粒附属物γ-微管蛋白在袖套成核中的作用。成熟精子中γ-微管蛋白的消失表明精子星体的形成应依赖于卵母细胞的γ-微管蛋白。一些人类精子中γ-微管蛋白的残余物表明父本γ-微管蛋白也可能有助于精子星体的形成。
