Toki T, Mori A, Shimizu M, Nikaido T, Fujii S
Department of Obstetrics and Gynecology, Shinshu University School of Medicine, Matsumoto, Japan.
Mol Hum Reprod. 1998 Dec;4(12):1157-64. doi: 10.1093/molehr/4.12.1157.
The aim of the present study was to gain a better understanding of the localization of apoptotic cells within the human endometrium during the menstrual cycle and to elucidate the relationships among the following for the human endometrium: apoptosis, p21 expression, and cell proliferation. Apoptosis and p21 expression were identified mainly in the glandular cells of the basal layer in the late secretory phase. In contrast, cells positive for Ki-67 were observed predominantly in the functional layer (in the proliferative phase in glandular cells and in the secretory phase in stromal cells). A very strong positive correlation (r = 0.81; P < 0.001) was demonstrated between the number of apoptotic cells and the number of p21-positive cells present among the glandular cells but, topographically, individual apoptotic cells were not coincident with p21-positive cells in serial sections. The results of this study suggest that the proliferation of the glandular cells of the basal layer is regulated by both apoptosis and p21 expression, particularly in the late secretory phase. Such regulation may be necessary to maintain a healthy population of glandular cells in the basal layer of the endometrium.
本研究的目的是更好地了解月经周期中人类子宫内膜内凋亡细胞的定位，并阐明人类子宫内膜中凋亡、p21表达和细胞增殖之间的关系。凋亡和p21表达主要在分泌晚期的基底层腺细胞中被发现。相比之下，Ki-67阳性细胞主要在功能层被观察到（增殖期腺细胞中以及分泌期基质细胞中）。在腺细胞中，凋亡细胞数量与p21阳性细胞数量之间显示出非常强的正相关（r = 0.81；P < 0.001），但是在连续切片中，单个凋亡细胞在地形学上与p21阳性细胞并不重合。本研究结果表明，基底层腺细胞的增殖受凋亡和p21表达两者调节，尤其是在分泌晚期。这种调节对于维持子宫内膜基底层腺细胞的健康群体可能是必要的。
