Campo J L, García Gil M
Area de Genética Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Madrid, Spain.
Poult Sci. 1998 Dec;77(12):1743-7. doi: 10.1093/ps/77.12.1743.
Tonic immobility reaction and heterophil: lymphocyte ratio (indices of fearfulness and stress, respectively) were studied in hens laying eggs with or without internal inclusions (blood and meat spots). Two brown egg Spanish breed of chickens were used. There were no significant differences between groups in terms of tonic immobility duration. Birds of the group with internal inclusions appeared to be less fearful when defined by the number of inductions to achieve tonic immobility (1.4+/-0.1 vs 1.1+/-0.1; P < 0.01). Tonic immobility duration was significantly shorter in the group of hens with internal inclusions, when only those laying the abnormal egg the day before measurement of tonic immobility were included in this group (264+/-33 vs 395+/-30 s; P < 0.01). There was a significant difference in the heterophil:lymphocyte ratio between the two groups of hens (P < 0.01), mean value being significantly higher in the group with internal inclusions (0.43+/-0.02) than in the control group (0.33+/-0.02). Hens that laid eggs with internal inclusions had significant heterophilia (P < 0.01). Differences were consistent across the breeds. The incidence of internal inclusions was significantly greater (P < 0.001) in eggs with pink shells (an abnormality associated with the effect of stress). The results suggest that the laying of eggs with internal inclusions involves changes in fearfulness and stressfulness of hens.
对产有或没有内部夹杂物（血斑和肉斑）鸡蛋的母鸡进行了强直静止反应和嗜异性粒细胞：淋巴细胞比率（分别为恐惧和应激指标）的研究。使用了两个西班牙褐壳蛋鸡品种。两组之间的强直静止持续时间没有显著差异。当根据达到强直静止的诱导次数来定义时，有内部夹杂物组的鸡似乎不那么恐惧（1.4±0.1对1.1±0.1；P<0.01）。当仅将在强直静止测量前一天产下异常蛋的母鸡纳入有内部夹杂物组时，该组的强直静止持续时间明显更短（264±33对395±30秒；P<0.01）。两组母鸡的嗜异性粒细胞：淋巴细胞比率存在显著差异（P<0.01），有内部夹杂物组的平均值（0.43±0.02）明显高于对照组（0.33±0.02）。产下有内部夹杂物鸡蛋的母鸡有明显的嗜异性粒细胞增多（P<0.01）。不同品种的差异是一致的。粉壳蛋（与应激效应相关的一种异常）中内部夹杂物的发生率显著更高（P<0.001）。结果表明，产下有内部夹杂物的鸡蛋涉及母鸡恐惧和应激性的变化。
