Park J S, Lee K R, Kim J C, Lim S H, Seo J A, Lee Y W
Division of Applied Biology and Chemistry and Research Center for New Biomaterials in Agriculture, Seoul National University, Suwon 441-744, Korea.
Appl Environ Microbiol. 1999 Jan;65(1):126-30. doi: 10.1128/AEM.65.1.126-130.1999.
Fifty-two isolates of Fusarium species were obtained from soybean seeds from various parts of Korea and identified as Fusarium oxysporum, F. moniliforme, F. semitectum, F. solani, F. graminearum, or F. lateritium. These isolates were grown on autoclaved wheat grains and examined for toxicity in a rat-feeding test. Nine cultures were toxic to rats. One of these, a culture of Fusarium sp. strain KCTC 16677, produced apicidin, an antiprotozoal agent that caused toxic effects in rats (including body weight loss; hemorrhage in the stomach, intestines, and bladder; and finally death) when rats were fed diets supplemented with 0.05 and 0.1% apicidin. The toxin was toxic to brine shrimp (the 50% lethal concentration was 40 microg/ml) and was weakly cytotoxic to human and mouse tumor cell lines.
从韩国各地的大豆种子中分离出52株镰刀菌，鉴定为尖孢镰刀菌、串珠镰刀菌、半裸镰刀菌、茄病镰刀菌、禾谷镰刀菌或砖红镰刀菌。将这些分离菌株接种于经高压灭菌的小麦粒上培养，并通过大鼠喂养试验检测其毒性。有9种培养物对大鼠有毒。其中一种，镰刀菌属菌株KCTC 16677的培养物产生了阿皮西丁，一种抗原生动物剂，当给大鼠喂食添加了0.05%和0.1%阿皮西丁的日粮时，会对大鼠产生毒性作用（包括体重减轻；胃、肠和膀胱出血；最终死亡）。该毒素对卤虫有毒（半数致死浓度为40微克/毫升），对人和小鼠肿瘤细胞系具有微弱的细胞毒性。
