Mukhopadhyay B, Johnson EF, Ascano M
Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.
Appl Environ Microbiol. 1999 Jan;65(1):301-6. doi: 10.1128/AEM.65.1.301-306.1999.
We describe a reactor-scale cultivation protocol for the fastest-growing and only known thermophilic member of the family Chlorobiaceae, Chlorobium tepidum. We discovered that C. tepidum would grow with sulfide as the sole electron source at rates and with final cell yields comparable to those found with thiosulfate only if the sulfide concentration was maintained below 0.1 mM and the culture redox potential was at -300 +/- 20 mV. Such was also the requirement for growth in a photobioreactor when thiosulfate (optimum level, 12 mM) was used as the preferred electron source. For cultivation of C. tepidum on a 5- to 500-ml scale, we used the system of Balch and Wolfe (Appl. Environ. Microbiol. 32:781-791, 1976) using stopper-sealed serum tubes and bottles as an alternative to the methods commonly used for the cultivation of phototrophic anaerobes and obtained consistent results.
我们描述了一种用于绿硫菌科生长最快且唯一已知的嗜热成员——嗜热绿硫菌（Chlorobium tepidum）的反应器规模培养方案。我们发现，只有当硫化物浓度保持在0.1 mM以下且培养物的氧化还原电位为-300±20 mV时，嗜热绿硫菌才能以硫化物作为唯一电子源生长，其生长速率和最终细胞产量与仅使用硫代硫酸盐时相当。当使用硫代硫酸盐（最佳水平为12 mM）作为首选电子源时，在光生物反应器中生长也是如此。对于5至500毫升规模的嗜热绿硫菌培养，我们使用了Balch和Wolfe的系统（《应用与环境微生物学》，第32卷，第781 - 791页，1976年），该系统使用带塞血清管和瓶子，作为培养光合厌氧菌常用方法的替代方法，并获得了一致的结果。
