Han Yuchen, Perner Mirjam
Molecular Biology of Microbial Consortia, University of Hamburg, Biocenter Klein Flottbek, Hamburg, Germany.
PLoS One. 2014 Aug 29;9(8):e106218. doi: 10.1371/journal.pone.0106218. eCollection 2014.
Sulfurimonas denitrificans was originally isolated from coastal marine sediments. It can grow with thiosulfate and nitrate or sulfide and oxygen. Recently sequencing of its genome revealed that it encodes periplasmic and cytoplasmic [NiFe]-hydrogenases but the role of hydrogen for its metabolism has remained unknown. We show the first experimental evidence that S. denitrificans can indeed express a functional hydrogen uptake active hydrogenase and can grow on hydrogen. In fact, under the provided conditions it grew faster and denser on hydrogen than on thiosulfate alone and even grew with hydrogen in the absence of reduced sulfur compounds. In our experiments, at the time points tested, the hydrogen uptake activity appeared to be related to the periplasmic hydrogenase and not to the cytoplasmic hydrogenase. Our data suggest that under the provided conditions S. denitrificans can grow more efficiently with hydrogen than with thiosulfate.
反硝化硫单胞菌最初是从沿海海洋沉积物中分离出来的。它可以利用硫代硫酸盐和硝酸盐或硫化物和氧气生长。最近对其基因组进行测序发现，它编码周质和细胞质[NiFe]氢化酶，但其代谢过程中氢气的作用仍不清楚。我们首次通过实验证明，反硝化硫单胞菌确实能够表达一种功能性的吸氢活性氢化酶，并能利用氢气生长。事实上，在给定条件下，它在氢气上生长得比仅在硫代硫酸盐上更快、更密集，甚至在没有还原态硫化合物的情况下也能利用氢气生长。在我们的实验中，在所测试的时间点，吸氢活性似乎与周质氢化酶有关，而与细胞质氢化酶无关。我们的数据表明，在给定条件下，反硝化硫单胞菌利用氢气生长比利用硫代硫酸盐更高效。
