关于重组猪α(1-3)半乳糖基转移酶的制备用途。
On the preparative use of recombinant pig alpha(1-3)galactosyl-transferase.
作者信息
Baisch G, Ohrlein R, Kolbinger F, Streiff M
机构信息
Novartis Pharma AG, Basle, Switzerland.
出版信息
Bioorg Med Chem Lett. 1998 Jun 16;8(12):1575-8. doi: 10.1016/s0960-894x(98)00266-2.
PMID:9873393
Abstract
A series of non-natural N-acyl derivatives of lactosamine is incubated with recombinant alpha(1-3)galactosyl-transferase and UDP-galactose. The enzyme shows a high promiscuity towards the non-natural acceptors. It selectively transfers a galactose unit onto the 3-OH group of the terminal beta-linked galactose in an alpha-mode to give an array of linear-B trisaccharides.
摘要
将一系列乳糖胺的非天然N-酰基衍生物与重组α(1-3)半乳糖基转移酶和UDP-半乳糖一起孵育。该酶对非天然受体表现出高度的混杂性。它以α-模式选择性地将一个半乳糖单元转移到末端β-连接的半乳糖的3-OH基团上，生成一系列线性-B三糖。
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